The effectivity of Centella asiatica extract on salivary neutrophils proliferation in severe early childhood caries

Dental caries, defined as multifactorial chronic disease, begins with the biofilm microbial complex change that is affected by sugar intake, salivary flow, and behavior. Neutrophils are the first line of defense against microbes. Besides kill microbes by phagocytosis, releases reactive oxygen species (ROS) and contains antimicrobial peptide, neutrophils activation also sets up the immune response. Saponin triterpenoids contained in Centella asiatica demonstrated immune-modulator effects, while pectin isolated from Centella asiatica showed immune-stimulatory activity. To analyze the effectivity of Centella asiatica extract as an immune-modulator in salivary neutrophil proliferation as innate immunity effector cells in severe early childhood caries. Preparation of extract of Centella asiatica were done using and isolation of neutrophils from the saliva of severe early childhood caries (S-ECC) using the magnitude of beads and marker label CD177 were analyzed using flow cytometry. Immune-modulator activity of Centella asiatica extract on salivary neutrophils proliferation was analyzed using MTT assay. Results: Salivary neutrophil proliferation increased after centella extract administration started from 50 μg/ ml. The extract of Centella asiatica effectively increase salivary neutrophil proliferation on administration 50 μg/ ml.