TY - JOUR
T1 - The Effectiveness of Epigallocatechin Gallate (EGCG) Eye Drops to Interleukin -1ß and Matrix Metalloproteinase (MMP)-9 Expression in Pseudomonas Aeruginosa Keratitis
T2 - An In Vitro Approach
AU - Urolita, Tarosa Yodia
AU - Nurwasis,
AU - Zuhria, Ismi
AU - Fathimah, Fariztah Sukainah Nur
AU - Karima, Annisa
AU - Kurniasari, Nila
AU - Purwanto, Djoko Agus
AU - Koendhori, Eko Budi
N1 - Publisher Copyright:
© 2024 by SPC (Sami Publishing Company)
PY - 2024/3
Y1 - 2024/3
N2 - Introduction: Pseudomonas aeruginosa keratitis is the most prevalent eye infection, known to potentially lead to endophthalmitis and corneal perforation. Although antibiotic therapy effectively eliminates the bacteria, the damage induced by toxins in the cornea persists, resulting in corneal thinning, perforation, or fibrosis. The healing process of the corneal wound is influenced by Interleukin-1β and matrix metalloproteinase-9 (MMP-9). Epigallocatechin gallate (EGCG), possessing antibacterial, anti-inflammatory, and antioxidant properties found in Camellia sinensis extract, holds promise as an adjuvant therapy for bacterial keratitis. This study aims to analyze the expression of Interleukin-1β and matrix metalloproteinase-9 (MMP-9) in a keratitis model induced by Pseudomonas aeruginosa infection following the administration of Epigallocatechin Gallate (EGCG) eye drops (an experimental study conducted on Rattus norvegicus rat models). Methods: The experimental study utilized samples of Rattus norvegicus corneas, previously induced to form keratitis models, with 6 samples each in the negative control group, Moxifloxacin group, and combined Moxifloxacin and EGCG group. Results: The percentage of Interleukin-1β expression in the combined EGCG and Moxifloxacin group was lower compared to the Moxifloxacin group. The results indicated a significant difference in the Interleukin-1β expression among the test groups (p < 0.05). The post hoc test revealed significant differences between the Moxifloxacin group and the combined EGCG group (p < 0.05), as well as between the negative control group and the combined EGCG group (p < 0.05). The mean percentage of MMP-9 expression in the combined EGCG group was lower than that in the Moxifloxacin group. The One-way ANOVA test indicated a significant difference among the three treatment groups (p < 0.05).
AB - Introduction: Pseudomonas aeruginosa keratitis is the most prevalent eye infection, known to potentially lead to endophthalmitis and corneal perforation. Although antibiotic therapy effectively eliminates the bacteria, the damage induced by toxins in the cornea persists, resulting in corneal thinning, perforation, or fibrosis. The healing process of the corneal wound is influenced by Interleukin-1β and matrix metalloproteinase-9 (MMP-9). Epigallocatechin gallate (EGCG), possessing antibacterial, anti-inflammatory, and antioxidant properties found in Camellia sinensis extract, holds promise as an adjuvant therapy for bacterial keratitis. This study aims to analyze the expression of Interleukin-1β and matrix metalloproteinase-9 (MMP-9) in a keratitis model induced by Pseudomonas aeruginosa infection following the administration of Epigallocatechin Gallate (EGCG) eye drops (an experimental study conducted on Rattus norvegicus rat models). Methods: The experimental study utilized samples of Rattus norvegicus corneas, previously induced to form keratitis models, with 6 samples each in the negative control group, Moxifloxacin group, and combined Moxifloxacin and EGCG group. Results: The percentage of Interleukin-1β expression in the combined EGCG and Moxifloxacin group was lower compared to the Moxifloxacin group. The results indicated a significant difference in the Interleukin-1β expression among the test groups (p < 0.05). The post hoc test revealed significant differences between the Moxifloxacin group and the combined EGCG group (p < 0.05), as well as between the negative control group and the combined EGCG group (p < 0.05). The mean percentage of MMP-9 expression in the combined EGCG group was lower than that in the Moxifloxacin group. The One-way ANOVA test indicated a significant difference among the three treatment groups (p < 0.05).
KW - Epigallocatechin gallate
KW - Interleukin-1β
KW - Keratitis
KW - MMP-9
UR - http://www.scopus.com/inward/record.url?scp=85179782179&partnerID=8YFLogxK
U2 - 10.26655/JMCHEMSCI.2024.3.1
DO - 10.26655/JMCHEMSCI.2024.3.1
M3 - Article
AN - SCOPUS:85179782179
SN - 2651-4702
VL - 7
SP - 461
EP - 473
JO - Journal of Medicinal and Chemical Sciences
JF - Journal of Medicinal and Chemical Sciences
IS - 3
ER -