The Effect ofBlack Adulsa LeafExtract on Glucose Tolerance and Pancreatic Islets ofMice

Listijani Suhargo, Diana Natalia Candra, Saikhu Ahmad Husain, Dwi Winarni

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

The leaves of black adulsa [Graptophyllum pictum (L.) Griff.] contained flavonoid that could bind to estrogen receptor and induced fat catabolism in ovariectomy condition with no estrogen production from ovary. Fat accumulation in ovariectomy condition could induce hyperglycemia. This research was aimed to determine the effect of black adulsa leaves ethanol extract on glucose tolerance and pancreatic islets of langerhans in ovariectomized mice. This research used 30 female mice divided into six groups, namely, the normal control group (non-ovariectomy, C I, 0.2 ml distilled water), the ovariectomized control group (C2, 0.2ml distilled water), the hormonal control groups (C3, 0,2 ml esthero solution in 0,08 mg/kg) and the treatment groups with the black adulsa leaves ethanol extract T I, T2 and T3 (10 mg/kg, 20 mg/kg and 30 mg/kg) respectively. After the end of the research all mice were tested for glucose tolerance, sacrificed and the pancreas organ was cut off to make pancreas slide with paraffin method and Hematoxyllin-Eosin staining. The glucose tolerance was calculated by A U C value. The result of this research showed that the black adulsa leaves ethanol extract decreased glucose tolerance and increase the diameter of pancreatic islet of Langerhans, the optimal dose was 20 mg/kg for mouse.

Original languageEnglish
Title of host publication8th International Conference and Workshop on Basic and Applied Science, ICOWOBAS 2021
EditorsAnjar Tri Wibowo, M. Fariz Fadillah Mardianto, Riries Rulaningtyas, Satya Candra Wibawa Sakti, Muhammad Fauzul Imron, Rico Ramadhan
PublisherAmerican Institute of Physics Inc.
ISBN (Electronic)9780735442610
DOIs
Publication statusPublished - 25 Jan 2022
Event8th International Conference and Workshop on Basic and Applied Science, ICOWOBAS 2021 - Surabaya, Indonesia
Duration: 25 Aug 202126 Aug 2021

Publication series

NameAIP Conference Proceedings
Volume2554
ISSN (Print)0094-243X
ISSN (Electronic)1551-7616

Conference

Conference8th International Conference and Workshop on Basic and Applied Science, ICOWOBAS 2021
Country/TerritoryIndonesia
CitySurabaya
Period25/08/2126/08/21

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