The leaves of black adulsa [Graptophyllum pictum (L.) Griff.] contained flavonoid that could bind to estrogen receptor and induced fat catabolism in ovariectomy condition with no estrogen production from ovary. Fat accumulation in ovariectomy condition could induce hyperglycemia. This research was aimed to determine the effect of black adulsa leaves ethanol extract on glucose tolerance and pancreatic islets of langerhans in ovariectomized mice. This research used 30 female mice divided into six groups, namely, the normal control group (non-ovariectomy, C I, 0.2 ml distilled water), the ovariectomized control group (C2, 0.2ml distilled water), the hormonal control groups (C3, 0,2 ml esthero solution in 0,08 mg/kg) and the treatment groups with the black adulsa leaves ethanol extract T I, T2 and T3 (10 mg/kg, 20 mg/kg and 30 mg/kg) respectively. After the end of the research all mice were tested for glucose tolerance, sacrificed and the pancreas organ was cut off to make pancreas slide with paraffin method and Hematoxyllin-Eosin staining. The glucose tolerance was calculated by A U C value. The result of this research showed that the black adulsa leaves ethanol extract decreased glucose tolerance and increase the diameter of pancreatic islet of Langerhans, the optimal dose was 20 mg/kg for mouse.