The effect of vitamin on wistar rats’ testicle s exposed tlethal insecticide

Gabriella Ayu Pitaloka, Kusnoto, Thomas Valentinus Widiyatno

Research output: Contribution to journalArticlepeer-review


Background: Agricultural products exposed to endosulfan insecticides potentially lead to organ dysfunction in living organisms that can cause morphological changes in the reproductive system such as a decrease in the number of sperm cells, an inhibition on the testosterone production by the Leydig cells, and a decline in the ascorbic acid levels in the testicles. Purpose: This study aimed to determine the effect of vitamin C on testicle weight and the number of Leydig cells in adult male Wistar rats exposed to endosulfan. Method: This study used 20 male Wistar rats with an average weight of 25 grams as samples, where the rats were equally divided into four groups, i.e., P-, P +, P1, and P2. The testicles of all Wistar rats were harvested on day 19 and then prepared for a microscopic examination with routine H & E staining. The results analyzed by using ANOVA and the Waller-Duncan T-test. Results: The testicle weight of the Wistar rats administered with endosulfan suggested a significant difference (p <0.05) and had the lowest value compared to the other treatments. The Wistar rats administered with endosulfan and then followed by vitamin C administration did not indicate a significant difference (p <0.05). Vitamin C administration to Wistar rats caused an increase in testicle weight. Conversely, administering endosulfan to the Wistar rats caused a decrease in testicle weight. Conclusion: Administering vitamin C to Wistar rats exposed to endosulfan could potentially lead to an increase in the testicle weight and the Leydig cells.

Original languageEnglish
Pages (from-to)1467-1471
Number of pages5
JournalIndian Journal of Public Health Research and Development
Issue number9
Publication statusPublished - Sept 2019


  • Endosulfan
  • Leydig cells
  • Testicle weight
  • Vitamin C
  • Wistar rats


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