The effect of various concentration of Bovine Serum Albumin in Mice (Mus musculus) In vitro Embryo culture on the number of Blastocyst

Research output: Contribution to journalArticlepeer-review

Abstract

This study aimed to determine the optimal concentration of Bovine Serum Albumin in increasing the amount of blastocyst. Oocytes were obtained from female mice Balb/C strains. Oocyte cells were collected and divided into 3 groups: T1 (MEM + BSA 3%), T2 (MEM + BSA 5%), and T3 (MEM + BSA 7%). Semen is collected from fertile male mice, then motile spermatozoa were put into a medium containing eggs. Media contained spermatozoa and eggs were stored in a 5% CO2
incubator. Observations were made under an inverted microscope at 100x magnification. Embryos that developed to the blastocyst stage show with a
clear cytoplasm, clear plasma membrane, no granularity, and clear pellucida zone. Based on statistical data analysis, there was a significant difference between the highest and lowest concentrations of p<0.05, with significant differences in the treatment group T1 (54.00a ± 8.94) and the treatment group T3 (32.00b ± 10.95). It can be concluded that the optimal concentration of Bovine Serum Albumin in supporting embryonic development is a concentration of 3%. The addition of Bovine Serum Albumin to the culture media was able to support the development of mouse embryos to the blastocyst stage.
Original languageEnglish
Article number43
Pages (from-to)1331
Number of pages1336
JournalEcology, Environment and Conservation
Volume27
Issue number3
Publication statusPublished - 2 Sept 2021

Keywords

  • Food production
  • Blastocyst
  • Bovine serum albumin
  • In vitro culture

Fingerprint

Dive into the research topics of 'The effect of various concentration of Bovine Serum Albumin in Mice (Mus musculus) In vitro Embryo culture on the number of Blastocyst'. Together they form a unique fingerprint.

Cite this