TY - JOUR
T1 - THE EFFECT OF USING A BOVINE PERICARDIUM SCAFFOLD ON THE GROWTH AND DIFFERENTIATION OF ADIPOSE-DERIVED MESENCHYMAL STEM CELLS (ADMSC)
AU - Soebroto, Heroe
AU - Mahyudin, Ferdiansyah
AU - Mastutik, Gondo
AU - Johan, Abu Rizal Dwikatmono
AU - Hariftyani, Arisvia Sukma
AU - Albab, Chabib Fachry
N1 - Publisher Copyright:
© 2024, Media Shed. All rights reserved.
PY - 2024/3
Y1 - 2024/3
N2 - Introduction: Decellularized pericardium seeded with adipose-derived mesenchymal stem cells is being developed in cardiac surgery to overcome closure difficulties and reduce adhesion risks. This study aims to explain the role of bovine pericardium scaffold as a medium for growth, proliferation and differentiation of ADMSC. Methods: The research is a true experimental type of research using in vitro tests through the posttest-only control group design. Variables studied included viability, TGF-B, NO, calretinin, Wilms Tumor I (WT I), vimentin, and FSP1. Results: The average live cell has a diameter of 23.56 µm, while the average dead cell has a cell diameter of 10.96 µm. The scaffold decellularized with ASB-16 had anti-inflammatory activity as indicated by the presence of TGF-β (p= 0.000164) and Nitric Oxide (NO) (p= 0.033031). It was detected the presence of mesothelial progenitors as indicated by the presence of the WT1 marker (p= 0.000374). The presence of mesothelium is indicated by the presence of the Calretinin (p= 0.003). The presence of fibroblast progenitors as indicated by the presence of the FSP1 (p= 0.021910). Fibroblasts as indicated by the detectable Vimentin marker (p= 0.122889). Conclusion: The bovine pericardium scaffold can be used as a growth medium for ADMSC to be able to proliferate and differentiate into mesothelial as evidenced by the presence of WT1 and Calretinin markers, and into fibroblasts as evidenced by the presence of FSP1 and Vimentin markers.
AB - Introduction: Decellularized pericardium seeded with adipose-derived mesenchymal stem cells is being developed in cardiac surgery to overcome closure difficulties and reduce adhesion risks. This study aims to explain the role of bovine pericardium scaffold as a medium for growth, proliferation and differentiation of ADMSC. Methods: The research is a true experimental type of research using in vitro tests through the posttest-only control group design. Variables studied included viability, TGF-B, NO, calretinin, Wilms Tumor I (WT I), vimentin, and FSP1. Results: The average live cell has a diameter of 23.56 µm, while the average dead cell has a cell diameter of 10.96 µm. The scaffold decellularized with ASB-16 had anti-inflammatory activity as indicated by the presence of TGF-β (p= 0.000164) and Nitric Oxide (NO) (p= 0.033031). It was detected the presence of mesothelial progenitors as indicated by the presence of the WT1 marker (p= 0.000374). The presence of mesothelium is indicated by the presence of the Calretinin (p= 0.003). The presence of fibroblast progenitors as indicated by the presence of the FSP1 (p= 0.021910). Fibroblasts as indicated by the detectable Vimentin marker (p= 0.122889). Conclusion: The bovine pericardium scaffold can be used as a growth medium for ADMSC to be able to proliferate and differentiate into mesothelial as evidenced by the presence of WT1 and Calretinin markers, and into fibroblasts as evidenced by the presence of FSP1 and Vimentin markers.
KW - ADMSC
KW - Bovine Pericardium
KW - Differentiation
KW - Growth
KW - Scaffold
UR - http://www.scopus.com/inward/record.url?scp=85188591610&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:85188591610
SN - 1462-2815
VL - 21
SP - 431
EP - 441
JO - Community Practitioner
JF - Community Practitioner
IS - 3
ER -