The effect of the endothelial nitric oxide synthase on hypoxia-induced factor-1 alpha level in a state of endothelial dysfunction after hyperbaric oxygen therapy

Aim: This research aims to know the effect of eNOS on hypoxia-induced factor-1 alpha (HIF-1α) levels in endothelial dysfunction after hyperbaric oxygen therapy (HBOT). Materials and Methods: The design of this study was experimental research; the study sample consisted of 30 Sprague Dawley (Rattus norvegicus) white rats and divided into three groups p1, p2, and p3; p1 rats with a standard diet, p2 rats with a high-cholesterol diet, and p3 rats with a high-cholesterol diet continued HBOT at 2.4 ATA with 98% O₂ for three sessions with a duration of 30 min/session, and air brakes for 5 min between each session for 10 consecutive days. eNOS and HIF-1α were examined using the enzyme-linked immunosorbent assay method. Results: From the results of testing the significance of the regression model, the results showed that there was a relation of HIF-1α on eNOS levels (P = 0.009) after treatment with HBO 2,4 ATA with 98% oxygen for three sessions with the duration of 30 min/session, and air brake for 5 min between each session for 10 days consecutively. Conclusion: The effect of eNOS on HIF-1α levels in HBOT shows that HBO can significantly affect the levels of HIF-1α through endothelial nitric oxide synthase (eNOS) level.