The effect of sodium hyaluronate to the properties of collagen-chitosan composite as artificial cornea-An in vitro study

Novi Dwi Widya Rini, Prihartini Widiyanti, Siswanto

Research output: Contribution to journalArticlepeer-review


The cornea is in the front of the eye that is prone to damage to the point of blindness. As many as 36 million people in the world suffer from blindness and it is estimated that this number will increase in double by 2020. Allogeneic grafts produce a rejection reaction in the patient's body up to 65% within 4 months of transplantation. An alternative to corneas with appropriate transmittance, morphological, and degradation characteristics needs to be sought. It is known that the addition of 0.5% w / v sodium hyaluronate (NaHA) to collagen 1% w / v + chitosan 2% w / v is capable of producing up to 95% transmittance percentages. This study aims to determine the effect of sodium hyaluronate on artificial corneal biocomposite transmittance and determine the morphological characteristics and artificial corneal biocomposite degradation. Artificial cornea was made from collagen, chitosan, and NaHA with a ratio of 20% w /v collagen, 10% w / v chitosan, and NaHA varied with a ratio of 0%; 0.3%; and 0.6% w /v. The artificial cornea was made by linking collagen with Hydroxypropyl methylcellulose (HPMC) which was mixed with chitosan and NaHA by using a magnetic stirrer. Then, heating was performed by using an incubator. Transmittance test results show that increasing the concentration of NaHA provides a transmittance value that is increasingly in accordance with the human cornea. The degradation test gives an artificially faster corneal degradation rate than the rate of human corneal degradation. Surface morphology test gives a picture of three samples that have a structure that is less strong and fragile. Based on this study, it was concluded that NaHA can increase artificial corneal transmittance, whereas the degradability and morphological nature of artificial corneal biocomposites need to be improved in further studies.

Original languageEnglish
Pages (from-to)1217-1222
Number of pages6
JournalJournal of International Dental and Medical Research
Issue number3
Publication statusPublished - 2020


  • Artificial cornea
  • Degradation
  • Morphology
  • Sodium hyaluronate
  • Transmittance


Dive into the research topics of 'The effect of sodium hyaluronate to the properties of collagen-chitosan composite as artificial cornea-An in vitro study'. Together they form a unique fingerprint.

Cite this