The effect of saltwater fish consumption by female house mice (Mus Musculus) on the increasing teeth enamel density of their pups: MicroCT analysis

Dental caries represent a common chronic condition the four main contributory factors of which are hosts, agents, substrates and time. One factor where an increase in tooth enamel density could reduce damage caused by the demineralization process. One saltwater biota which contains various proteins having a function in supporting enamel maturation is saltwater fish. A Radiography Micro Computed Tomography Scanner, commonly known as a μ-CT Scanner, is a device used to quantify the increase in tooth enamel density. This study aims to examine the effect of saltwater fish powder on the ability of female mice to increase teeth enamel density in their pups. This study type was true experimental in character using the Completely Randomized Design. 24 impregnated mice (Musmusculus)used as samples which were then given saltwater fish powder derived from sardines (Sardinellafimbriata), splendid ponyfish (Leiognathussplendens) and cobs (Euthynnusaffinis). The mice were divided into three concentration groups: group 1 (1.07 mg/0.5 ml), group 2 (2.14 mg/0.5 ml) and the control group. Samples were observed with a SkyScan1173 Scanner, while the enamel density was measured using a GrayScale indicator and analyzed by means of a One Way Anova test. The results confirmed a significant difference between the enamel density in the control group with that of group 1 and group 2, whereas no significant difference existed between group 1 and group 2. There was an increase in tooth enamel density of those mice pups belonging to groups whose mothers had consumed saltwater fish.