TY - JOUR
T1 - The effect of okra pods (Abelmoschus esculentus l.) methanol extract on white blood cell count, phagocytic activity, and ifn-γ level in mus musculus exposed sodium nitrite
AU - Wahyuningsih, Sri Puji Astuti
AU - Isnaini, Siti Richa
AU - Winarni, Dwi
AU - Darmanto, Win
AU - Pramudya, Manikya
AU - Nafi’ah, Anni Mar’Atun
AU - Khoirunnisa,
AU - Sajidah, Elma Sakinatus
AU - Atika, Baiq Naili Dewi
N1 - Publisher Copyright:
© EM International.
PY - 2020
Y1 - 2020
N2 - Okra is widely used as a vegetable and known have health benefits because it contains high antioxidants. The purpose of this study was to determine antioxidant potential of okra pods methanol extract (OPM) on immune response (white blood cell count, activity phagocytic, and IFN-γ level) of Mus musculus exposed sodium nitrite (NaNO 2). Twenty four male mice (BALB/c strain, 8-10 weeks, 25-30 g body weight were divided into 6 groups: normal control group (exposed distilled water), negative control (exposed NaNO 2 50 mg/kg body weight), and OPM treatment (exposed to NaNO2 and OPM 50, 100, 200 and 400 mg/kg BW simultaneously). The mice was administrated orally both NaNO2 and OPM once a day for 19 days. Finally treatment, the parameter analysis was performed statistically at P = 0.05. The results showed that the administration of OPM could increase white blood cell count (WBCs), phagocytic activity, and IFN-γ level significantly. The optimal dose of OPM is 50 mg/kg BW. The conclusion of this study is that the administration of OPM can increase the immune response (white blood cell count, activity phagocytic, and IFN-γ levels) of Mus musculus exposed NaNO2.
AB - Okra is widely used as a vegetable and known have health benefits because it contains high antioxidants. The purpose of this study was to determine antioxidant potential of okra pods methanol extract (OPM) on immune response (white blood cell count, activity phagocytic, and IFN-γ level) of Mus musculus exposed sodium nitrite (NaNO 2). Twenty four male mice (BALB/c strain, 8-10 weeks, 25-30 g body weight were divided into 6 groups: normal control group (exposed distilled water), negative control (exposed NaNO 2 50 mg/kg body weight), and OPM treatment (exposed to NaNO2 and OPM 50, 100, 200 and 400 mg/kg BW simultaneously). The mice was administrated orally both NaNO2 and OPM once a day for 19 days. Finally treatment, the parameter analysis was performed statistically at P = 0.05. The results showed that the administration of OPM could increase white blood cell count (WBCs), phagocytic activity, and IFN-γ level significantly. The optimal dose of OPM is 50 mg/kg BW. The conclusion of this study is that the administration of OPM can increase the immune response (white blood cell count, activity phagocytic, and IFN-γ levels) of Mus musculus exposed NaNO2.
KW - Blood
KW - IFN-γ level
KW - Okra pods
KW - Sodium nitrite
UR - http://www.scopus.com/inward/record.url?scp=85090399837&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:85090399837
SN - 0971-765X
VL - 26
SP - S41-S48
JO - Ecology, Environment and Conservation
JF - Ecology, Environment and Conservation
ER -