TY - JOUR
T1 - The effect of long-term ketogenic diet on serum adiponectin and insulin-like growth factor-1 levels in mice
AU - Widiatmaja, Deandra M.
AU - Lutvyani, Alif
AU - Sari, Desi R.
AU - Kurniasari, Hamidah
AU - Meiliana, Ismi D.
AU - Fasitasari, Minidian
AU - Yamaoka, Yoshio
AU - Rejeki, Purwo S.
N1 - Funding Information:
This work was funded by the Directorate of Research and Community Service, Deputy for Strengthening Research and Development, Ministry of Research and Technology/National Research and Innovation Agency (582/UN3.14/PT/2020).
Funding Information:
The authors would like to thank the Directorate of Research and Community Service, Deputy for Strengthening Research and Development, Ministry of Research and Technology/National Research and Innovation Agency for funding this study. The authors would also like to thank all the parties of Laboratory Animals of Biochemistry, Faculty of Medicine, Universitas Airlangga who have helped the experimental study.
Funding Information:
Research funding: This work was funded by the Directorate of Research and Community Service, Deputy for Strengthening Research and Development, Ministry of Research and Technology/National Research and Innovation Agency (582/UN3.14/PT/2020).
Publisher Copyright:
© 2021 Walter de Gruyter GmbH, Berlin/Boston.
PY - 2022/9/1
Y1 - 2022/9/1
N2 - Objectives: Noncommunicable disease (NCD) including obesity, cancer, and diabetes has become particular concern worldwide due to its morbidity and mortality which keep increasing annually. Adiponectin and insulin-like growth factor-1 (IGF-1) are known to be substances that are involved in the development of NCD. Several diet regimens have been developed to treat NCD, one of which is the ketogenic diet (KD). This study aimed to analyze the long-term KD effect on serum adiponectin and IGF-1 levels in mice. Methods: This study was a real experimental with post-test only controls group design. The subjects were 14 male mice (2-3 months, 20-30 g) were randomly divided into two groups, K1 (n=7, standard diet) and K2 (n=7, KD with a composition of 60% fat, 30% protein, and 10% fiber). All subjects were given diet intervention for 8 weeks ad libitum. Serum adiponectin and IGF levels were measured in post-intervention using Enzyme-Linked Immunosorbent Assay. Distribution of normality was analyzed by the Shapiro-Wilk Test, mean difference using Independent T-Test, and linear correlation using Pearson's Correlation Test. Data analysis was performed using Statistic Package for Social Science Version 16. Results: Serum adiponectin levels in K1 (0.080 ± 0.012) pg/mL and K2 (0.099 ± 0.005) pg/mL, with p=0.003. Serum IGF-1 levels in K1 (133.535 ± 25.702) ng/mL and K2 (109.987 ± 27.118) ng/mL, with p=0.121. Coefficient correlation between serum adiponectin and serum IGF-1 levels [r]=-0.401, with p=0.155. Conclusions: Long-term KD increases serum adiponectin levels and has no effect on serum IGF-1 levels. There was no significant correlation between serum adiponectin and serum IGF-1 levels.
AB - Objectives: Noncommunicable disease (NCD) including obesity, cancer, and diabetes has become particular concern worldwide due to its morbidity and mortality which keep increasing annually. Adiponectin and insulin-like growth factor-1 (IGF-1) are known to be substances that are involved in the development of NCD. Several diet regimens have been developed to treat NCD, one of which is the ketogenic diet (KD). This study aimed to analyze the long-term KD effect on serum adiponectin and IGF-1 levels in mice. Methods: This study was a real experimental with post-test only controls group design. The subjects were 14 male mice (2-3 months, 20-30 g) were randomly divided into two groups, K1 (n=7, standard diet) and K2 (n=7, KD with a composition of 60% fat, 30% protein, and 10% fiber). All subjects were given diet intervention for 8 weeks ad libitum. Serum adiponectin and IGF levels were measured in post-intervention using Enzyme-Linked Immunosorbent Assay. Distribution of normality was analyzed by the Shapiro-Wilk Test, mean difference using Independent T-Test, and linear correlation using Pearson's Correlation Test. Data analysis was performed using Statistic Package for Social Science Version 16. Results: Serum adiponectin levels in K1 (0.080 ± 0.012) pg/mL and K2 (0.099 ± 0.005) pg/mL, with p=0.003. Serum IGF-1 levels in K1 (133.535 ± 25.702) ng/mL and K2 (109.987 ± 27.118) ng/mL, with p=0.121. Coefficient correlation between serum adiponectin and serum IGF-1 levels [r]=-0.401, with p=0.155. Conclusions: Long-term KD increases serum adiponectin levels and has no effect on serum IGF-1 levels. There was no significant correlation between serum adiponectin and serum IGF-1 levels.
KW - IGF-1
KW - adiponectin
KW - ketogenic diet
KW - long-term
KW - mice
KW - obesity
UR - http://www.scopus.com/inward/record.url?scp=85118272471&partnerID=8YFLogxK
U2 - 10.1515/jbcpp-2021-0287
DO - 10.1515/jbcpp-2021-0287
M3 - Article
AN - SCOPUS:85118272471
SN - 0792-6855
VL - 33
SP - 611
EP - 618
JO - Journal of Basic and Clinical Physiology and Pharmacology
JF - Journal of Basic and Clinical Physiology and Pharmacology
IS - 5
ER -