TY - JOUR
T1 - The Effect of Lipoteichoic Acid from Lactobacillus plantarum on Dental Pulp Inflammation
AU - Pribadi, Nirawati
AU - Rahayu, Retno Pudji
AU - Ismiyatin, Kun
AU - Putri, Cindy Ramadhan
AU - Surboyo, Meircurius Dwi Condro
N1 - Publisher Copyright:
© 2021. European Journal of Dentistry.
PY - 2021/10/1
Y1 - 2021/10/1
N2 - Objective Lactobacillus plantarum, a bacterium located in deep caries, has a virulence factor in the form of lipoteichoic acid (LTA), which is found in the bacterial cell wall. LTA is able to trigger a neutrophils response in the dental pulp inflammation process within the first 6 to 24 hours. The quantity of bacteria is one factor influencing the increase in number of neutrophils in addition to the quality of the bacteria. This study seeks to analyze the effect of lipoteichoic acid of Lactobacillus plantarum (LTA-Lp) in the dental pulp inflammation by observing the number of neutrophil cells in a histopathological view. Materials and Methods The LTA was isolated from L. plantarum. The left upper molar of Rattus novergicus was mechanically perforated under anesthesia to induce dental pulp inflammation. The perforated tooth was then induced by 10 and 15 μg/mL of LTA-Lp and then restored by a temporary filling. The perforated tooth in the control group was only restored by a temporary filling. After 24, 48, and 72 hours, the tooth was extracted and then stained with hematoxylins and eosin to observe the neutrophils in the dental pulp via a light microscope. Result The number of neutrophils in the dental pulp after induction by 15 μg/mL of LTA-Lp is higher than 10 μg/mL of LTA-Lp and both controls. There were significant differences in the number of neutrophils in the dental pulp, in each group on 24, 48, and 72 hours after LTA-Lp inducing (p < 0.05). Conclusion The LTA-Lp dose of of 10 and 15 μg/mL affected the dental pulp inflammation by affecting the number of neutrophils.
AB - Objective Lactobacillus plantarum, a bacterium located in deep caries, has a virulence factor in the form of lipoteichoic acid (LTA), which is found in the bacterial cell wall. LTA is able to trigger a neutrophils response in the dental pulp inflammation process within the first 6 to 24 hours. The quantity of bacteria is one factor influencing the increase in number of neutrophils in addition to the quality of the bacteria. This study seeks to analyze the effect of lipoteichoic acid of Lactobacillus plantarum (LTA-Lp) in the dental pulp inflammation by observing the number of neutrophil cells in a histopathological view. Materials and Methods The LTA was isolated from L. plantarum. The left upper molar of Rattus novergicus was mechanically perforated under anesthesia to induce dental pulp inflammation. The perforated tooth was then induced by 10 and 15 μg/mL of LTA-Lp and then restored by a temporary filling. The perforated tooth in the control group was only restored by a temporary filling. After 24, 48, and 72 hours, the tooth was extracted and then stained with hematoxylins and eosin to observe the neutrophils in the dental pulp via a light microscope. Result The number of neutrophils in the dental pulp after induction by 15 μg/mL of LTA-Lp is higher than 10 μg/mL of LTA-Lp and both controls. There were significant differences in the number of neutrophils in the dental pulp, in each group on 24, 48, and 72 hours after LTA-Lp inducing (p < 0.05). Conclusion The LTA-Lp dose of of 10 and 15 μg/mL affected the dental pulp inflammation by affecting the number of neutrophils.
KW - dental pulp
KW - lactobacillus plantarum
KW - lipoteichoic acid
KW - neutrophils
KW - pulp inflammation
UR - http://www.scopus.com/inward/record.url?scp=85113884712&partnerID=8YFLogxK
U2 - 10.1055/s-0041-1728238
DO - 10.1055/s-0041-1728238
M3 - Article
AN - SCOPUS:85113884712
SN - 1305-7456
VL - 15
SP - 682
EP - 686
JO - European Journal of Dentistry
JF - European Journal of Dentistry
IS - 4
ER -