TY - GEN
T1 - The Effect of Human Umbilical Cord Blood- Mesenchymal Stem Cells-Derived Secretome on the Proliferation and Migration of Endothelial Progenitor Cells
AU - Oktaviono, Yudi Her
AU - Sandra, Ferry
AU - Hutomo, Suryo Ardi
AU - Pramudita, Christian
AU - Isaridha, Ilma Alfia
AU - Susanti, Melly
AU - Hidayat, Dwi Fachrul Octafian
AU - Al-Farabi, Makhyan Jibril
N1 - Publisher Copyright:
© 2021, Springer Nature Switzerland AG.
PY - 2021
Y1 - 2021
N2 - Endothelial progenitor cells (EPCs) play an important role in the pathophysiology of coronary artery disease (CAD). Secretome produced by human Umbilical Cord Blood-Mesenchymal Stem Cells (hUCB-MSCs) shown to have neovascularization and angiogenesis effect. However, its impact toward EPCs proliferation and migration is not yet elucidated. This study aims to determine the effect of hUCB-MSCs-derived secretome on EPCs proliferation and migration capability. EPCs were isolated from peripheral blood samples of one CAD patient and cultured in the Stemline II medium. Cultured EPCs were placed in 6-well plates until it reached confluence and incubated with hUCB-MSCs-derived secretome at a concentration of 2%, 10%, and 20% (v/v). EPCs proliferation was determined using the XTT assay. Migration of EPCs was evaluated using a Boyden chamber assay. Statistical analysis was done using ANOVA test to compare between groups and correlation between variables was obtained using Spearman correlation test. EPCs proliferation at secretome dose 10% and 20% were 1.5–1.8 times higher than the control group (p < 0.05) while EPCs migration was superior at all concentrations (5–15 times higher, p < 0.05) compared to the control group. hUCB-MSCs-derived secretome increase EPCs proliferation and migration in a dose-dependent manner. This study shows that hUCB-MSCs-derived secretome increase EPCs proliferation and migration, making it a potential regenerative therapy for CAD patients.
AB - Endothelial progenitor cells (EPCs) play an important role in the pathophysiology of coronary artery disease (CAD). Secretome produced by human Umbilical Cord Blood-Mesenchymal Stem Cells (hUCB-MSCs) shown to have neovascularization and angiogenesis effect. However, its impact toward EPCs proliferation and migration is not yet elucidated. This study aims to determine the effect of hUCB-MSCs-derived secretome on EPCs proliferation and migration capability. EPCs were isolated from peripheral blood samples of one CAD patient and cultured in the Stemline II medium. Cultured EPCs were placed in 6-well plates until it reached confluence and incubated with hUCB-MSCs-derived secretome at a concentration of 2%, 10%, and 20% (v/v). EPCs proliferation was determined using the XTT assay. Migration of EPCs was evaluated using a Boyden chamber assay. Statistical analysis was done using ANOVA test to compare between groups and correlation between variables was obtained using Spearman correlation test. EPCs proliferation at secretome dose 10% and 20% were 1.5–1.8 times higher than the control group (p < 0.05) while EPCs migration was superior at all concentrations (5–15 times higher, p < 0.05) compared to the control group. hUCB-MSCs-derived secretome increase EPCs proliferation and migration in a dose-dependent manner. This study shows that hUCB-MSCs-derived secretome increase EPCs proliferation and migration, making it a potential regenerative therapy for CAD patients.
KW - Cardiovascular regenerative therapy
KW - Endothelial progenitor cells
KW - Migration
KW - Proliferation
KW - Stem cells
KW - hUCB-MSCs-derived secretome
UR - http://www.scopus.com/inward/record.url?scp=85097602264&partnerID=8YFLogxK
U2 - 10.1007/978-3-030-64610-3_70
DO - 10.1007/978-3-030-64610-3_70
M3 - Conference contribution
AN - SCOPUS:85097602264
SN - 9783030646097
T3 - IFMBE Proceedings
SP - 622
EP - 630
BT - 8th European Medical and Biological Engineering Conference - Proceedings of the EMBEC 2020
A2 - Jarm, Tomaz
A2 - Cvetkoska, Aleksandra
A2 - Mahnič-Kalamiza, Samo
A2 - Miklavcic, Damijan
PB - Springer Science and Business Media Deutschland GmbH
T2 - 8th European Medical and Biological Engineering Conference, EMBEC 2020
Y2 - 29 November 2020 through 3 December 2020
ER -
