The effect of gel abelmoschus manihot (L.) medik leaf extract on second degree burn wound healing process

Background: World Health Organization (WHO) estimated around 265000 of death is caused by burn injury. Abelmoschus manihot (L.) Medik consists of active compound such as hibifolin, stigmasterol, γ-sitosterol, myricetin, cannabistrin, myricetin 3-O-beta-D-glucopyranoside, 2,4-Dihydroxy benzoic zcid, asam maleat, quercetin, guanosine, adenosine. Material and methods: Thirty male rats (Rattus norvegicus) weighting 200g were divided into 5 experimental groups (T0, T1, T2, T3 and T4). The skin sample was observed by using histopathology preparation and blood sample was also observed. Results: Density of collagen on T0 showed significant difference to T1 and T2 (p<0,05), but P0 didn't show significant difference to T3 and T4 (p> 0,05). Arrangement of collagen on T0 showed significant difference to T1, T2 and T4 (p< 0,05), but no significant difference to T3 (p> 0,05). Based on statistical analysis fibroblast showed that P0 has no significant difference to T3 (p >0,05) but has significant difference to T1, T2, T4 (p<0,05). Conclusion: The skin evaluation that consists of density and arrangement of collagen and total of fibroblast shows that T3 is the best concentration of extract Abelmoschus manihot (L.) Medik leaf that can be used as a topical therapy for burn injury.