The Effect of Diallyl Trisulfide Administration on The Viability of MDA-MB-231 Cell Lines

Diallyl trisulfide or DATS is an important organic sulphur compound (OSCs) of garlic that has been recognized for its ability to inhibit migration and invasion processes, promote programmed cell death, and impede the proliferation of breast cancer cells. However, the most effective dosage of DATS to decrease survival rate and cell viability of triple-negative breast cancer cell line has been undetermined. The present study aimed to discover the MDA-MB-231 (triple-negative breast cancer cell line) viability differences due to the addition of DATS in several doses. DATS was administered to 6 groups of MDA-MB-231 cell cultures at different concentrations (20, 40, 80, 160, and 320 μM), with a control group. Cell viability was evaluated using colorimetric MTT assay. The study reported that DATS effectively inhibited the cell line survival rate. Treatment with DATS at a dose of 20 μM or higher led to cell death and a significant MDA-MB-231 cell line viability reduction compared to the control group but not between the intervention groups. The findings demonstrated that DATS had the ability to trigger cell death and possess potential anti-cancer properties particularly in triple-negative breast cancer. A novel observation was made when low dose of DATS exhibited comparable efficacy to the large dose in reducing MDA-MB-231 cell line viability, while minimizing the potential for negative side effects.