THE EFFECT OF COMBINATION CALCIUM HYDROXIDE AND PROPOLIS APPLICATION TO NUMBER OF NEUTROPHIL CELLS ON WISTAR RAT PULP PERFORATION

As the gold standard, pulp inflammation can be treated with pulp capping by calcium hydroxide. Tissue necrosis accompanied by inflammation could be caused by calcium hydroxide pulp capping. Calcium hydroxide combined with propolis which have anti-inflammatory effects through inhibition of the nf-kb pathway and pro-inflammatory cytokines. The increase in vascular permeability and neutrophil chemotaxis will be inhibited by that method. By regulating the quantity of neutrophil cells as an acute phase marker, the inflammatory process will speed up, so the healing process happens more quickly. The quantity of neutrophil cells in the pulp perforation of Wistar rats after calcium hydroxide-propolis combination application must therefore be examined. The aim of this study is to analyze the differencenumber of neutrophil cells onthe Wistar rats pulp perforation after applicated by calcium hydroxide-propolis combination compared with calcium hydroxide-aquadest. M1 tooth perforated for all groups with three types tratment: no medication (control group), calcium hydroxide-aquadest 1:1 (P-1 group), and calcium hydroxide-propolis 1:1.5 (P-2 group) and closed with cention. Observations were made on the 1st and 3rd day with HE staining. One Way ANOVA shown a significant difference (p <0.05) of the average amount of neutrophil cells between all treatment groups on 1st day (p = 0.000) and 3rd day (p = 0.000). In the calcium hydroxide-propolis (P-2) combination group, the number of neutrophil cells was lower than in the control group and in the calcium hydroxide-aquadest (P-1) group on days 1 and 3. The quantity of neutrophil cells after calcium hydroxide-propolis is added is smaller than that of calcium hydroxide-aquadest.