The effect of chitosan addition on cellular uptake and cytotoxicity of ursolic acid niosomes

Andang Miatmoko; Berlian S. Hariawan; Devy M. Cahyani; Retno Sari; Aristika Dinaryanti; Eryk Hendrianto

doi:10.1590/0001-3765202120201850

The effect of chitosan addition on cellular uptake and cytotoxicity of ursolic acid niosomes

Andang Miatmoko, Berlian S. Hariawan, Devy M. Cahyani, Retno Sari, Aristika Dinaryanti, Eryk Hendrianto

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

Abstract

This study evaluated the cellular uptake and cytotoxicity of low permeable Ursolic acid (UA) on cancer cells using niosomes composed of span 60 and cholesterol. The results showed that the addition of chitosan increased particle sizes and ζ-potentials. The UA niosomes with chitosan layers had higher cytotoxicity in HeLa cells than without chitosan, however, there was no improvement observed for Huh7it cells. Moreover, chitosan layers improved the cellular uptake, which clathrin-mediated endocytosis may determine the cellular transport of UA niosomes. In conclusion, the addition of chitosan improved cellular uptake and cytotoxicity of UA niosomes in the HeLa cells.

Original language	English
Article number	e20201850
Journal	Anais da Academia Brasileira de Ciencias
Volume	93
Issue number	3
DOIs	https://doi.org/10.1590/0001-3765202120201850
Publication status	Published - 2021

Keywords

Cancer
Cell uptake
Chitosan
Cytotoxicity
Niosomes
Ursolic acid

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1590/0001-3765202120201850

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title = "The effect of chitosan addition on cellular uptake and cytotoxicity of ursolic acid niosomes",

abstract = "This study evaluated the cellular uptake and cytotoxicity of low permeable Ursolic acid (UA) on cancer cells using niosomes composed of span 60 and cholesterol. The results showed that the addition of chitosan increased particle sizes and ζ-potentials. The UA niosomes with chitosan layers had higher cytotoxicity in HeLa cells than without chitosan, however, there was no improvement observed for Huh7it cells. Moreover, chitosan layers improved the cellular uptake, which clathrin-mediated endocytosis may determine the cellular transport of UA niosomes. In conclusion, the addition of chitosan improved cellular uptake and cytotoxicity of UA niosomes in the HeLa cells.",

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T1 - The effect of chitosan addition on cellular uptake and cytotoxicity of ursolic acid niosomes

AU - Miatmoko, Andang

AU - Hariawan, Berlian S.

AU - Cahyani, Devy M.

AU - Sari, Retno

AU - Dinaryanti, Aristika

AU - Hendrianto, Eryk

PY - 2021

Y1 - 2021

N2 - This study evaluated the cellular uptake and cytotoxicity of low permeable Ursolic acid (UA) on cancer cells using niosomes composed of span 60 and cholesterol. The results showed that the addition of chitosan increased particle sizes and ζ-potentials. The UA niosomes with chitosan layers had higher cytotoxicity in HeLa cells than without chitosan, however, there was no improvement observed for Huh7it cells. Moreover, chitosan layers improved the cellular uptake, which clathrin-mediated endocytosis may determine the cellular transport of UA niosomes. In conclusion, the addition of chitosan improved cellular uptake and cytotoxicity of UA niosomes in the HeLa cells.

AB - This study evaluated the cellular uptake and cytotoxicity of low permeable Ursolic acid (UA) on cancer cells using niosomes composed of span 60 and cholesterol. The results showed that the addition of chitosan increased particle sizes and ζ-potentials. The UA niosomes with chitosan layers had higher cytotoxicity in HeLa cells than without chitosan, however, there was no improvement observed for Huh7it cells. Moreover, chitosan layers improved the cellular uptake, which clathrin-mediated endocytosis may determine the cellular transport of UA niosomes. In conclusion, the addition of chitosan improved cellular uptake and cytotoxicity of UA niosomes in the HeLa cells.

KW - Cancer

KW - Cell uptake

KW - Chitosan

KW - Cytotoxicity

KW - Niosomes

KW - Ursolic acid

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DO - 10.1590/0001-3765202120201850

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JO - Anais da Academia Brasileira de Ciencias

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IS - 3

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ER -

The effect of chitosan addition on cellular uptake and cytotoxicity of ursolic acid niosomes

Abstract

Keywords

UN SDGs

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