The Effect of Calcium Hydroxide and Chitosan Combination as A Pulp Capping Material on The Increased Expression of Osteopontin and Runt-Related Transcription Factor 2 in Dentine Reparative Formation in Osteoporosis

Dian Agustin Wahjuningrum, Ravishinta Efty Arwinda, Veronica Regina Rosselle, Setyabudi, Tamara Yuanita, Anuj Bhardwaj, Novaldy Wahjudianto, Michelle Callea, Cruz González Alberto Carlos

Research output: Contribution to journalArticlepeer-review

Abstract

Direct pulp capping is performed to maintain the vitality of the exposed pulp by applying a dressing material to facilitate reparative dentinogenesis as a biological seal. Osteoporosis increases oxidative stress and inflammation therefore dentinogenesis was disrupted. Dentin bridge reparative produced by direct pulp capping material still inappropriate, there is a tunnel defect. Chitosan is a natural polysaccharide biopolymer, non-toxic, biocompatible, biodegradable, has broad spectrum antimicrobial properties, anti-inflammatory and antioxidant. OPN and RUNX2 secretion expressed by odontoblast-like cells can be a sign of reparative dentin formation in osteoporosis. The aim of this research to analyse the effect of calcium hydroxide and chitosan combination on the increased expression of OPN and RUNX2 on day 1, day 3 and day 6 in reparative dentin formation in osteoporosis. This experimental study using a total of 42 wistar rat. Ovariohysterectomy was performed to induced osteoporosis and verified by tunel assay after 3 months. Rats divided into 6 groups, the control group on day 1, day 3 and day 6, the treatment group on day 1, day 3 and day 6. Necropsy was performed and teeth samples were obtained. After being decalcified, specimens underwent anatomical histopathology evaluation under light microscope to determine the direction of the perforation. Immunohistochemical examination was performed to determine the odontoblast like cells that express OPN and RUNX2. The results were a significant increase in the expression of OPN and RUNX2 in the combination of calcium hydroxide with chitosan compared to the control group in osteoporosis. The conclusion is in osteoporosis, the expression of OPN and RUNX2 in the combination of calcium hydroxide with chitosan on day 1, day 3 and day 6 was higher than calcium hydroxide.

Original languageEnglish
Pages (from-to)1395-1399
Number of pages5
JournalJournal of International Dental and Medical Research
Volume16
Issue number4
Publication statusPublished - 2023

Keywords

  • Calcium hydroxide
  • Immunology
  • chitosan
  • pulp capping
  • reparative dentin

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