TY - JOUR
T1 - The Effect of Calcium Hydroxide and Chitosan Combination as A Pulp Capping Material on The Increased Expression of Osteopontin and Runt-Related Transcription Factor 2 in Dentine Reparative Formation in Osteoporosis
AU - Wahjuningrum, Dian Agustin
AU - Arwinda, Ravishinta Efty
AU - Rosselle, Veronica Regina
AU - Setyabudi,
AU - Yuanita, Tamara
AU - Bhardwaj, Anuj
AU - Wahjudianto, Novaldy
AU - Callea, Michelle
AU - Carlos, Cruz González Alberto
N1 - Publisher Copyright:
© (2023), (University of Dicle). All Rights Reserved.
PY - 2023
Y1 - 2023
N2 - Direct pulp capping is performed to maintain the vitality of the exposed pulp by applying a dressing material to facilitate reparative dentinogenesis as a biological seal. Osteoporosis increases oxidative stress and inflammation therefore dentinogenesis was disrupted. Dentin bridge reparative produced by direct pulp capping material still inappropriate, there is a tunnel defect. Chitosan is a natural polysaccharide biopolymer, non-toxic, biocompatible, biodegradable, has broad spectrum antimicrobial properties, anti-inflammatory and antioxidant. OPN and RUNX2 secretion expressed by odontoblast-like cells can be a sign of reparative dentin formation in osteoporosis. The aim of this research to analyse the effect of calcium hydroxide and chitosan combination on the increased expression of OPN and RUNX2 on day 1, day 3 and day 6 in reparative dentin formation in osteoporosis. This experimental study using a total of 42 wistar rat. Ovariohysterectomy was performed to induced osteoporosis and verified by tunel assay after 3 months. Rats divided into 6 groups, the control group on day 1, day 3 and day 6, the treatment group on day 1, day 3 and day 6. Necropsy was performed and teeth samples were obtained. After being decalcified, specimens underwent anatomical histopathology evaluation under light microscope to determine the direction of the perforation. Immunohistochemical examination was performed to determine the odontoblast like cells that express OPN and RUNX2. The results were a significant increase in the expression of OPN and RUNX2 in the combination of calcium hydroxide with chitosan compared to the control group in osteoporosis. The conclusion is in osteoporosis, the expression of OPN and RUNX2 in the combination of calcium hydroxide with chitosan on day 1, day 3 and day 6 was higher than calcium hydroxide.
AB - Direct pulp capping is performed to maintain the vitality of the exposed pulp by applying a dressing material to facilitate reparative dentinogenesis as a biological seal. Osteoporosis increases oxidative stress and inflammation therefore dentinogenesis was disrupted. Dentin bridge reparative produced by direct pulp capping material still inappropriate, there is a tunnel defect. Chitosan is a natural polysaccharide biopolymer, non-toxic, biocompatible, biodegradable, has broad spectrum antimicrobial properties, anti-inflammatory and antioxidant. OPN and RUNX2 secretion expressed by odontoblast-like cells can be a sign of reparative dentin formation in osteoporosis. The aim of this research to analyse the effect of calcium hydroxide and chitosan combination on the increased expression of OPN and RUNX2 on day 1, day 3 and day 6 in reparative dentin formation in osteoporosis. This experimental study using a total of 42 wistar rat. Ovariohysterectomy was performed to induced osteoporosis and verified by tunel assay after 3 months. Rats divided into 6 groups, the control group on day 1, day 3 and day 6, the treatment group on day 1, day 3 and day 6. Necropsy was performed and teeth samples were obtained. After being decalcified, specimens underwent anatomical histopathology evaluation under light microscope to determine the direction of the perforation. Immunohistochemical examination was performed to determine the odontoblast like cells that express OPN and RUNX2. The results were a significant increase in the expression of OPN and RUNX2 in the combination of calcium hydroxide with chitosan compared to the control group in osteoporosis. The conclusion is in osteoporosis, the expression of OPN and RUNX2 in the combination of calcium hydroxide with chitosan on day 1, day 3 and day 6 was higher than calcium hydroxide.
KW - Calcium hydroxide
KW - Immunology
KW - chitosan
KW - pulp capping
KW - reparative dentin
UR - http://www.scopus.com/inward/record.url?scp=85182220055&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:85182220055
SN - 1309-100X
VL - 16
SP - 1395
EP - 1399
JO - Journal of International Dental and Medical Research
JF - Journal of International Dental and Medical Research
IS - 4
ER -