The effect of alpha-mangostin on interleukin-10 and collagen 1A1 gene in the inflammation process: An experimental in-vitro study

Aims: Bone resorption after tooth extraction can cause serious problems on subsequent denture implants and restorative dentistry procedures that rely on the bone healing process. The peel of the mangostin fruit is one of the natural components that can help with wound healing. Its constituents, particularly alpha-mangostin, have antifungal, antioxidant, antiviral, antibacterial, and anti-inflammatory properties. The research was carried out because there was an effort to speed up the healing process following tooth extraction and because of the high potential of alpha-mangostin. The aim of this study is to see whether taking alpha-mangostin decreases interleukin-10 (IL-10) and increases collagen 1A1 expression in the inflammation process. Materials and Methods: This study was a post-test-only control group design. An in-vitro experiment was conducted on the following four groups. Then lipopolysaccharide (LPS) and alpha-mangostin were inducted into cell culture. In osteoblast cell culture 7F2, real-time polymerase chain reaction was performed to see markers collagen 1A1 and IL-10. The research was statistically analyzed, using a variance test and one-way analysis of variance. Results: The highest IL-10 gene expression was found in group induction with LPS, but there was no significant difference in IL-10 expression between the groups. The highest collagen 1A1 gene expression was found in a group that received induction with LPS and alpha-mangostin, but there was not a significant difference in collagen 1A1 expression between the groups. Conclusion: Alpha-mangostin induction effectively reduces inflammation and IL-10 expression, while increasing collagen 1A1 expression.