The differential expression of mgl mRNA by Porphyromonas gingivalis affects the production of methyl mercaptan

Kazuhisa Ouhara; Y. Iwasaki; M. Kajiya; I. Savitri; M. Kitagawa; N. Tokunaga; T. Shintani; I. Ogawa; T. Hino; T. Fujita; H. Shiba; H. Kurihara

doi:10.1111/odi.12326

The differential expression of mgl mRNA by Porphyromonas gingivalis affects the production of methyl mercaptan

Kazuhisa Ouhara, Y. Iwasaki, M. Kajiya, I. Savitri, M. Kitagawa, N. Tokunaga, T. Shintani, I. Ogawa, T. Hino, T. Fujita, H. Shiba, H. Kurihara

Research output: Contribution to journal › Article › peer-review

7 Citations (Scopus)

Abstract

Objective: A large number of individuals have halitosis. The total amount of volatile sulfur compounds, which are the main cause of halitosis, has been correlated with periodontitis following bacterial infection. In this study, Porphyromonas gingivalis (Pg), a major periodontopathogenic bacterium, was isolated from patients with halitosis by the amplification of 16S rRNA, and the ability of isolated Pg to produce methyl mercaptan (CH₃SH) was determined to clarify the relationship between halitosis and Pg infection. Materials and Methods: CH₃SH concentrations were measured in patients using Oral Chroma. The production of CH₃SH by Pg standard and clinical strains was also measured in vitro. Real-time PCR was performed to compare the expression of mgl mRNA (which encoded l-methionine-a-deamino-g-mercaptomethane-lyase) among the Pg strains. The production of CH₃SH and the expression of mgl mRNA were also determined to assess the effects of oriental medicine. Results: The production of CH₃SH and the expression of mgl mRNA strongly correlated with each other in the presence of l-methionine. The expression of mgl mRNA by Pg W83 was strongly inhibited by magnoliaceae. Conclusion: The production of CH₃SH was correlated with the expression of mgl. Furthermore, the oriental medicine, magnoliaceae, may represent a potential treatment for halitosis.

Original language	English
Pages (from-to)	626-633
Number of pages	8
Journal	Oral Diseases
Volume	21
Issue number	5
DOIs	https://doi.org/10.1111/odi.12326
Publication status	Published - 1 Jul 2015
Externally published	Yes

Keywords

Halitosis
Periodontitis
Porphyromonas gingivalis
Volatile sulfur compounds

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10.1111/odi.12326

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title = "The differential expression of mgl mRNA by Porphyromonas gingivalis affects the production of methyl mercaptan",

abstract = "Objective: A large number of individuals have halitosis. The total amount of volatile sulfur compounds, which are the main cause of halitosis, has been correlated with periodontitis following bacterial infection. In this study, Porphyromonas gingivalis (Pg), a major periodontopathogenic bacterium, was isolated from patients with halitosis by the amplification of 16S rRNA, and the ability of isolated Pg to produce methyl mercaptan (CH3SH) was determined to clarify the relationship between halitosis and Pg infection. Materials and Methods: CH3SH concentrations were measured in patients using Oral Chroma. The production of CH3SH by Pg standard and clinical strains was also measured in vitro. Real-time PCR was performed to compare the expression of mgl mRNA (which encoded l-methionine-a-deamino-g-mercaptomethane-lyase) among the Pg strains. The production of CH3SH and the expression of mgl mRNA were also determined to assess the effects of oriental medicine. Results: The production of CH3SH and the expression of mgl mRNA strongly correlated with each other in the presence of l-methionine. The expression of mgl mRNA by Pg W83 was strongly inhibited by magnoliaceae. Conclusion: The production of CH3SH was correlated with the expression of mgl. Furthermore, the oriental medicine, magnoliaceae, may represent a potential treatment for halitosis.",

keywords = "Halitosis, Periodontitis, Porphyromonas gingivalis, Volatile sulfur compounds",

author = "Kazuhisa Ouhara and Y. Iwasaki and M. Kajiya and I. Savitri and M. Kitagawa and N. Tokunaga and T. Shintani and I. Ogawa and T. Hino and T. Fujita and H. Shiba and H. Kurihara",

note = "Publisher Copyright: {\textcopyright} 2015 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.",

year = "2015",

month = jul,

day = "1",

doi = "10.1111/odi.12326",

language = "English",

volume = "21",

pages = "626--633",

journal = "Oral Diseases",

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TY - JOUR

T1 - The differential expression of mgl mRNA by Porphyromonas gingivalis affects the production of methyl mercaptan

AU - Ouhara, Kazuhisa

AU - Iwasaki, Y.

AU - Kajiya, M.

AU - Savitri, I.

AU - Kitagawa, M.

AU - Tokunaga, N.

AU - Shintani, T.

AU - Ogawa, I.

AU - Hino, T.

AU - Fujita, T.

AU - Shiba, H.

AU - Kurihara, H.

PY - 2015/7/1

Y1 - 2015/7/1

N2 - Objective: A large number of individuals have halitosis. The total amount of volatile sulfur compounds, which are the main cause of halitosis, has been correlated with periodontitis following bacterial infection. In this study, Porphyromonas gingivalis (Pg), a major periodontopathogenic bacterium, was isolated from patients with halitosis by the amplification of 16S rRNA, and the ability of isolated Pg to produce methyl mercaptan (CH3SH) was determined to clarify the relationship between halitosis and Pg infection. Materials and Methods: CH3SH concentrations were measured in patients using Oral Chroma. The production of CH3SH by Pg standard and clinical strains was also measured in vitro. Real-time PCR was performed to compare the expression of mgl mRNA (which encoded l-methionine-a-deamino-g-mercaptomethane-lyase) among the Pg strains. The production of CH3SH and the expression of mgl mRNA were also determined to assess the effects of oriental medicine. Results: The production of CH3SH and the expression of mgl mRNA strongly correlated with each other in the presence of l-methionine. The expression of mgl mRNA by Pg W83 was strongly inhibited by magnoliaceae. Conclusion: The production of CH3SH was correlated with the expression of mgl. Furthermore, the oriental medicine, magnoliaceae, may represent a potential treatment for halitosis.

AB - Objective: A large number of individuals have halitosis. The total amount of volatile sulfur compounds, which are the main cause of halitosis, has been correlated with periodontitis following bacterial infection. In this study, Porphyromonas gingivalis (Pg), a major periodontopathogenic bacterium, was isolated from patients with halitosis by the amplification of 16S rRNA, and the ability of isolated Pg to produce methyl mercaptan (CH3SH) was determined to clarify the relationship between halitosis and Pg infection. Materials and Methods: CH3SH concentrations were measured in patients using Oral Chroma. The production of CH3SH by Pg standard and clinical strains was also measured in vitro. Real-time PCR was performed to compare the expression of mgl mRNA (which encoded l-methionine-a-deamino-g-mercaptomethane-lyase) among the Pg strains. The production of CH3SH and the expression of mgl mRNA were also determined to assess the effects of oriental medicine. Results: The production of CH3SH and the expression of mgl mRNA strongly correlated with each other in the presence of l-methionine. The expression of mgl mRNA by Pg W83 was strongly inhibited by magnoliaceae. Conclusion: The production of CH3SH was correlated with the expression of mgl. Furthermore, the oriental medicine, magnoliaceae, may represent a potential treatment for halitosis.

KW - Halitosis

KW - Periodontitis

KW - Porphyromonas gingivalis

KW - Volatile sulfur compounds

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U2 - 10.1111/odi.12326

DO - 10.1111/odi.12326

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C2 - 25703825

AN - SCOPUS:84930271142

SN - 1354-523X

VL - 21

SP - 626

EP - 633

JO - Oral Diseases

JF - Oral Diseases

IS - 5

ER -

The differential expression of mgl mRNA by Porphyromonas gingivalis affects the production of methyl mercaptan

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Keywords

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