Several efforts have been made to discover new anticancer agents based on natural ingredients. Meanwhile, previous studies have shown that different Pine genus species exhibit cytotoxic activity against various types of cancer cells. This plant is rich in phenolic compounds, especially procyanidins, flavonoids, and phenolic acids. Therefore, this study aims to investigate the in vitro cytotoxicity of Pinus merkusii needles extract on HeLa cancer cell lines. The cytotoxicity assessment was measured using MTT assay and expressed as IC50 value. The results showed that the ethanolic extract poses a dose and time-dependent cytotoxic activity with an IC50 value of 542.5 µg/ml at 48 hours of incubation. Based on this result, Pinus merkusii needles' ethanolic extract has the potential of a novel candidate for an anticancer agent.

Original languageEnglish
Article number03001
JournalBIO Web of Conferences
Publication statusPublished - 23 Aug 2021
Event1st International Conference of Advanced Veterinary Science and Technologies for Sustainable Development, ICAVESS 2021 - Virtual, Online, Indonesia
Duration: 28 Mar 202129 Mar 2021


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