The composition of the amino acids protein ghrelin and neuropeptide y as the basis for regulating energy balance in broilers

The purpose of this study was to determine the molecular weight of the protein ghrelin as a basis to determine the amino acid composition of protein ghrelin and subsequently to make synthetic ghrelin protein whose function is to control energy balance in broilers. Samples isolated from the digestive tract and brain tissue of the broilers and then examined by SDS Page and the Western blot test. Based on the results, it can be concluded that the protein ghrelin had a molecular weight of 44 kDa and composed of methionine, phenylalanine, leucine, arginine, valine, isoleucine, and leucine.