Introduction. Alveolar bone defect in dentistry can be caused by injury after tooth extraction, periodontal disease, enucleation of a cyst, and tumor surgery. Scaffold in tissue engineering is an important material that can stimulate osteogenesis process. Lymphocyte cells have a role in promoting and accelerating the proliferation of supporting cells like osteoblast to accelerate the bone regeneration process. Objective. The purpose of this study was to determine the effect of chitosan-collagen chicken shank collagen used as scaffold for bone regeneration through lymphocyte cell proliferation. Method. Twelve Wistar rats (Rattus norvegicus) were prepared as animal models in this study. Bone defects are intentionally made in both the right and left femur bones of the rat. Total samples were 24 divided into four groups: Group 1 as a control Group using 3% CMC-Na, Group 2 using chitosan scaffold only, Group 3 using chitosan-chicken shank collagen scaffold (50:50), and Group 4 using chitosan-chicken shank collagen scaffold (80:20). The animals were sacrificed on the 5th day, and histopathological examination was carried out to observe the number of lymphocyte cells. Results. Significant differences between all groups can be showed in the one-way ANOVA test (p value>0.05). The highest lymphocyte cells were found in Group 3 with chitosan-chicken shank collagen scaffold (50:50). Conclusion. The chitosan-chicken shank collagen used as scaffold can increase the bone regeneration process through increased lymphocyte cell proliferation.

Original languageEnglish
Pages (from-to)43-48
Number of pages6
JournalActa Medica Philippina
Issue number8
Publication statusPublished - 2022


  • bone regeneration
  • chicken shank collagen
  • chitosan
  • lymphocyte
  • scaffold


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