TY - JOUR
T1 - The Benefits of the Combination of Vitamin D3, K2 Supplements, and UV-B Exposure for Increasing Bone Density
T2 - A Simple Solution for Bone Health
AU - Nelwan, Sindy Cornelia
AU - Tedjosasongko, Udijanto
AU - Kuntari, Satiti
AU - Utomo, Daniel Haryono
AU - Saskianti, Tania
AU - Puteri, Mega Moeharyono
AU - Dharmawan, Devi
AU - Fitriani, Yufita
AU - Kurnia, Pradita Agung
AU - Mufida, Amalia Ramadhani
AU - Fajrin, Nadhia Zahria
AU - Rahayu, Retno Pudji
AU - Nowwarote, Nunthawan
N1 - Funding Information:
The authors would like to thank the Pediatric Dentistry Department, Faculty of Dental Medicine, and Biochemistry Department of the Faculty of Medicine at Universitas Airlangga for their technical support to this study. The authors would also like to express our gratitude to the Directorate of Research, Technology, and Higher Education of the Republic of Indonesia for giving us research grant with Grant Number of 623/UN3.14/PT/202O.
Publisher Copyright:
© 2021, Journal of International Dental and Medical Research. All Rights Reserved.
PY - 2021
Y1 - 2021
N2 - Bones and teeth require nutrients to maintain optimal bone density. Environmental and habitual factors affect 20-40% of bone density in addition to several nutrients such as vitamin D, especially for UV-B exposures which are essential for bone growth. Research on the impact of nutrition on bone strength has historically geared towards minerals, vitamin D, protein, and vitamin K. This study aimed to examine the role of vitamin D3, K2 supplements, and UV-B exposure on bone density, reflected by osteoblast cell formation. Pre and post-experimental laboratory studies were conducted to Wistar rats (Rattus norvegicus), which were divided into six groups. As much as 0.00045 mg/day of vitamin D and 0.81 mcg/day of K2 were administered. Besides, 290-315 nm of UV-B was administered 3 times a week for 25 minutes each. With the HE (hematoxylin eosin) test, the level of osteoblast cell was calculated after 21 days of treatment. Descriptive test results showed that the highest mean score was obtained from the vitamin D3+ K2 group. The ANOVA test result showed sig < 0.05 (p < 0.05), which indicated a significant difference. This research shows that the combination of vitamin D3 and K2 significantly increased osteoblast cell formation.
AB - Bones and teeth require nutrients to maintain optimal bone density. Environmental and habitual factors affect 20-40% of bone density in addition to several nutrients such as vitamin D, especially for UV-B exposures which are essential for bone growth. Research on the impact of nutrition on bone strength has historically geared towards minerals, vitamin D, protein, and vitamin K. This study aimed to examine the role of vitamin D3, K2 supplements, and UV-B exposure on bone density, reflected by osteoblast cell formation. Pre and post-experimental laboratory studies were conducted to Wistar rats (Rattus norvegicus), which were divided into six groups. As much as 0.00045 mg/day of vitamin D and 0.81 mcg/day of K2 were administered. Besides, 290-315 nm of UV-B was administered 3 times a week for 25 minutes each. With the HE (hematoxylin eosin) test, the level of osteoblast cell was calculated after 21 days of treatment. Descriptive test results showed that the highest mean score was obtained from the vitamin D3+ K2 group. The ANOVA test result showed sig < 0.05 (p < 0.05), which indicated a significant difference. This research shows that the combination of vitamin D3 and K2 significantly increased osteoblast cell formation.
KW - Bone density
KW - Human and health
KW - Osteoblast
KW - Vitamin d3
KW - Vitamin k2
UR - http://www.scopus.com/inward/record.url?scp=85123862434&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:85123862434
SN - 1309-100X
VL - 14
SP - 1453
EP - 1458
JO - Journal of International Dental and Medical Research
JF - Journal of International Dental and Medical Research
IS - 4
ER -
