TY - JOUR
T1 - The antidiabetic effect of bitter melon (Momordica charantia l.) extracts towards glucose concentration, langerhans islets, and leydig cells of hyperglycemic mice (rattus norvegicus)
AU - Meles, Dewa Ketut
AU - Wurlina,
AU - Adnyana, Dewa Putu Anom
AU - Rinaldhi, Chaterina Puspadewanti
AU - Octaviani, Rian Rizky
AU - Cempaka, Desak Ketut Sekar
N1 - Funding Information:
Author thanks to Brawijaya University and Airlangga University for supporting this research.
Publisher Copyright:
© 2019 Meles et al.
PY - 2019/8/1
Y1 - 2019/8/1
N2 - Purpose: This study was aimed to prove the fruit extract of bitter melon (Momordica charantia L.) towards blood sugar levels, of Langerhans islets cells, and Leydig cells of hyperglycemic white mice (Rattus norvegicus). Methods: Twenty-five mice were divided randomly into 5 groups. They all were induced intraperitoneally by alloxan with dosage of 150 mg/kg in order to damage to the pancreas. From all treatments group, three groups (P1, P2, and P3) were treated with various doses of bitter melon extract with dosage 29, 50, and 59 mg/1 ml/day, respectively. As a comparative group, –negative control group (P0 +) were given with CMC-Na 0,5% 1ml/day, whereas the positive control group (K+) were given Glibenclamide® 0.126 mg/1 ml/day. Bitter melon extract was given for 21 days. In the first day of treatment, blood glucose level of mice was examined after 2 hours, 4 hours, 6 hours, 8 hours after treatments. The blood glucose examinations were subsequently continued at days 7th, 14th and 21st after treatment. After 21 days, the pancreas and testes of mice were taken for histopathological preparations made. Results: Bitter melon (Momordica charantia L.) extract had antidiabetic effects that can lower blood glucose level, improved pancreatic beta cell damage, and increased the Leydig cells number in a dosage of 50 mg/1 ml/day on the 21st days after treatment. Conclusion: The extract of bitter melon fruit (Momordica charantia L.) at a dosage of 50 mg/kg/1ml/day can lower blood glucose levels and increased the number of Langerhans islets and Leydig cell of hyperglycemia mice.
AB - Purpose: This study was aimed to prove the fruit extract of bitter melon (Momordica charantia L.) towards blood sugar levels, of Langerhans islets cells, and Leydig cells of hyperglycemic white mice (Rattus norvegicus). Methods: Twenty-five mice were divided randomly into 5 groups. They all were induced intraperitoneally by alloxan with dosage of 150 mg/kg in order to damage to the pancreas. From all treatments group, three groups (P1, P2, and P3) were treated with various doses of bitter melon extract with dosage 29, 50, and 59 mg/1 ml/day, respectively. As a comparative group, –negative control group (P0 +) were given with CMC-Na 0,5% 1ml/day, whereas the positive control group (K+) were given Glibenclamide® 0.126 mg/1 ml/day. Bitter melon extract was given for 21 days. In the first day of treatment, blood glucose level of mice was examined after 2 hours, 4 hours, 6 hours, 8 hours after treatments. The blood glucose examinations were subsequently continued at days 7th, 14th and 21st after treatment. After 21 days, the pancreas and testes of mice were taken for histopathological preparations made. Results: Bitter melon (Momordica charantia L.) extract had antidiabetic effects that can lower blood glucose level, improved pancreatic beta cell damage, and increased the Leydig cells number in a dosage of 50 mg/1 ml/day on the 21st days after treatment. Conclusion: The extract of bitter melon fruit (Momordica charantia L.) at a dosage of 50 mg/kg/1ml/day can lower blood glucose levels and increased the number of Langerhans islets and Leydig cell of hyperglycemia mice.
KW - Blood sugar levels
KW - Hyperglycemia
KW - Leydig cells
KW - Melon extract
KW - Pancreas cells
UR - http://www.scopus.com/inward/record.url?scp=85071388177&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:85071388177
SN - 1307-9867
VL - 13
SP - 757
EP - 762
JO - EurAsian Journal of BioSciences
JF - EurAsian Journal of BioSciences
IS - 2
ER -