TY - JOUR
T1 - The anti-inflammatory activity of p-methoxycinnamic acid (PMCA) in the nanostructured lipid carrier (NLC) system using combinations of solid lipid, beeswax-oleum cacao and liquid lipid, virgin coconut oil (VCO)
AU - Erawati, Tristiana
AU - Hariyadi, Dewi Melani
AU - Rosita, Noorma
AU - Purwanti, Tutiek
N1 - Publisher Copyright:
© RJPT All right reserved.
PY - 2019/8
Y1 - 2019/8
N2 - Objective: The nanostructured lipid carrier (NLC) system used the combination of beeswax-oleum cacao and virgin coconut oil (VCO) in this study, it was the development of nanoemulsion (NE) and solid lipid nanoparticle (SLN) delivery systems, which are used as nano-carriers of p-methoxycinnamic acid (PMCA). PMCA a secunder metabolite of champheria galanga has antiinflamatory effect which is difficult to dissolve in water. Several combinations of solid lipids (beeswax-oleum cacao) and liquid lipids (VCO) were prepared, namely F I (60:40); F II (70:30); and F III (80:20) in the NLC system. Material and Method: The NLC-PMCA system was prepared by the high shear homogenization method, then characterized and subjected to anti-inflammatory activity tests compared with nanoemulsion and solid lipid nanoparticle (SLN). The results of the research: The increasing concentration of VCO in the NLC-PMCA system resulted in lower viscosity, and greater entrapment efficiency, namely in the NLC-PMCA system with a combination of beeswax-oleum cacao and VCO of 60:40. NLC-PMCA with the combination of beeswax-oleum cacao and VCO of 60:40 (F I) has particle size (236.00 ± 17.15 nm) smaller than SLN-PMCA (665.60 ± 72.59 nm) but biger than NE-PMCA (29.27 ± 1.85 nm). Occlucivity of F I NLC-PMCA (41.50 ± 4.00 %) higher than SLN-PMCA (33.52 ± 3.65 %) and NE-PMCA (33.52 ± 3.65 %). PMCA in F I (60: 40) NLC, SLN and NE has the equivalent anti-inflammatory activity based on the observations of the PMN cells number in ear skin mice with edema. Conclusion: The NLC-PMCA with the combination of beeswax-oleum cacao and VCO of 60:40 was the best formula.
AB - Objective: The nanostructured lipid carrier (NLC) system used the combination of beeswax-oleum cacao and virgin coconut oil (VCO) in this study, it was the development of nanoemulsion (NE) and solid lipid nanoparticle (SLN) delivery systems, which are used as nano-carriers of p-methoxycinnamic acid (PMCA). PMCA a secunder metabolite of champheria galanga has antiinflamatory effect which is difficult to dissolve in water. Several combinations of solid lipids (beeswax-oleum cacao) and liquid lipids (VCO) were prepared, namely F I (60:40); F II (70:30); and F III (80:20) in the NLC system. Material and Method: The NLC-PMCA system was prepared by the high shear homogenization method, then characterized and subjected to anti-inflammatory activity tests compared with nanoemulsion and solid lipid nanoparticle (SLN). The results of the research: The increasing concentration of VCO in the NLC-PMCA system resulted in lower viscosity, and greater entrapment efficiency, namely in the NLC-PMCA system with a combination of beeswax-oleum cacao and VCO of 60:40. NLC-PMCA with the combination of beeswax-oleum cacao and VCO of 60:40 (F I) has particle size (236.00 ± 17.15 nm) smaller than SLN-PMCA (665.60 ± 72.59 nm) but biger than NE-PMCA (29.27 ± 1.85 nm). Occlucivity of F I NLC-PMCA (41.50 ± 4.00 %) higher than SLN-PMCA (33.52 ± 3.65 %) and NE-PMCA (33.52 ± 3.65 %). PMCA in F I (60: 40) NLC, SLN and NE has the equivalent anti-inflammatory activity based on the observations of the PMN cells number in ear skin mice with edema. Conclusion: The NLC-PMCA with the combination of beeswax-oleum cacao and VCO of 60:40 was the best formula.
KW - Anti-inflammatory activity
KW - Beeswax
KW - Nanostructured lipid carrier (NLC)
KW - Oleum cacao
KW - P-methoxycinnamic acid (PMCA)
KW - Virgin coconut oil (VCO)
UR - http://www.scopus.com/inward/record.url?scp=85075898426&partnerID=8YFLogxK
U2 - 10.5958/0974-360X.2019.00617.6
DO - 10.5958/0974-360X.2019.00617.6
M3 - Article
AN - SCOPUS:85075898426
SN - 0974-3618
VL - 12
SP - 3619
EP - 3625
JO - Research Journal of Pharmacy and Technology
JF - Research Journal of Pharmacy and Technology
IS - 8
ER -