TY - GEN
T1 - The Analysis of Phage rz/rz1 Gene in Shigatoxin Bacteria
AU - Sjahriani, Tessa
AU - Arivo, Debi
AU - Wasito, Eddy Bagus
AU - Tyasningsih, Wiwiek
N1 - Publisher Copyright:
© 2022 ACM.
PY - 2022/5/15
Y1 - 2022/5/15
N2 - The phage rz/rz1 gene is a membrane subunit that is required for phage lysis to breach the bacterial host's outer membrane. Because of their proclivity to excise and infect multiple hosts, they are key drivers of horizontal gene transfer resulting in the bacteria becoming a STEC. This study aim to looked at the rz/rz1 gene of E. coli strain STEC and Shigella sonnei carrying stx gene, yielded eight lytic bacteriophages, using PCR, DNA sequencing and NCBI Blast, Expasy translation and Expasy protparam for analyze. The rz/rz1 gene sequences were found to have 97.73 % to 98.90 % identical similarities with E. coli strain STEC and Shigella sonnei. According to the Kruskal-Wallis statistical test, there was a significant difference in rz/rz1 amino acid between Phage, E. coli strain STEC and Shigella sonnei (p < 0.001). The conclusion is the rz/rz1 gene of lytic bacteriophage was involved in the spread of stx gene, with significant difference of amino acid.
AB - The phage rz/rz1 gene is a membrane subunit that is required for phage lysis to breach the bacterial host's outer membrane. Because of their proclivity to excise and infect multiple hosts, they are key drivers of horizontal gene transfer resulting in the bacteria becoming a STEC. This study aim to looked at the rz/rz1 gene of E. coli strain STEC and Shigella sonnei carrying stx gene, yielded eight lytic bacteriophages, using PCR, DNA sequencing and NCBI Blast, Expasy translation and Expasy protparam for analyze. The rz/rz1 gene sequences were found to have 97.73 % to 98.90 % identical similarities with E. coli strain STEC and Shigella sonnei. According to the Kruskal-Wallis statistical test, there was a significant difference in rz/rz1 amino acid between Phage, E. coli strain STEC and Shigella sonnei (p < 0.001). The conclusion is the rz/rz1 gene of lytic bacteriophage was involved in the spread of stx gene, with significant difference of amino acid.
KW - E. coli
KW - Shigella sonnei
KW - lytic phage
KW - rz/rz1
KW - stx
UR - http://www.scopus.com/inward/record.url?scp=85140038374&partnerID=8YFLogxK
U2 - 10.1145/3545729.3545777
DO - 10.1145/3545729.3545777
M3 - Conference contribution
AN - SCOPUS:85140038374
T3 - ACM International Conference Proceeding Series
SP - 238
EP - 241
BT - ICMHI 2022 - 2022 6th International Conference on Medical and Health Informatics
PB - Association for Computing Machinery
T2 - 6th International Conference on Medical and Health Informatics, ICMHI 2022
Y2 - 12 May 2022 through 15 May 2022
ER -