Synthesis, characterization and evaluation of swelling ratio on magnetic p53-poly(MAA-co-EGDMA)@GO-Fe3O4(MIP@GO-Fe3O4)-based p53 protein and graphene oxide from kusambi wood (Schleichera oleosa)

Apris A. Adu, Yantus A.B. Neolaka, Arsel A.Pau Riwu, Munawar Iqbal, Handoko Darmokoesoemo, Heri Septya Kusuma

Research output: Contribution to journalArticlepeer-review

23 Citations (Scopus)

Abstract

The p53 protein plays an important role by acting as a transcription factor to regulate geneexpression and its genetic mutation has been reported as the most common genetic alter-ations in human cancer diseases. This opens possibilities for the development of earlyidentification of cancer by utilizing p53 protein as tumor biomarkers. In this work wereport the synthesis, characterization and evaluation of p53-poly(MAA-co-EGDMA)@GO-Fe3O4as SPE materials for selective extraction of wild p53 protein. The magnetic materialp53-poly(MAA-co-EGDMA)@GO-Fe3O4(MIP@GO-Fe3O4) is successfully prepared with GO-Fe3O4from kusambi wood (Schleichera oleosa) as the host material, p53 as a templatemolecule, methacrylic acid (MAA) as a monomer, ethylene glycol dimethacrylate (EGDMA)as a crosslinker, benzoyl peroxide (BPO) as an initiator and acetone/ethanol as a porogen.Synthesis is carried out using a bulk polymerization method. To find out the best ratiothat produces the highest MIP@GO-Fe3O4mass, the five variations of the volume ratio oftemplate, monomer and crosslinker (P1-P5) are investigated. It was found that the vol-ume ratio of template, monomer and crosslinker that produced the highest MIP@GO-Fe3O4mass is obtained at P3 volume ratio (1:4:8 mL, which is further polymerized with GO-Fe3O4as much as 0.6239 g). Swelling ratio studies using 3 (three) solvents namely chloroform,acetone and methanol show that the swelling ratio values obtained are 1.46, 1.24 and 1.23 g/g, respectively. These results indicate that methanol is the best solvent for use in the rebind- ing process of MIP@GO-Fe3O4 material. Based on the analysis by FTIR, XRD, SEM-EDX and BET-BJH, the resulting MIP@GO-Fe3O4 material is expected to be used later for wild p53 preconcentration from various biological samples.

Original languageEnglish
Pages (from-to)11060-11068
Number of pages9
JournalJournal of Materials Research and Technology
Volume9
Issue number5
DOIs
Publication statusPublished - 2020

Keywords

  • Bulk polymerization
  • Graphene oxide
  • Imprinting polymer
  • Schleichera oleosa
  • Wild p53 protein

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