TY - JOUR
T1 - Synthesis, characterization and evaluation of swelling ratio on magnetic p53-poly(MAA-co-EGDMA)@GO-Fe3O4(MIP@GO-Fe3O4)-based p53 protein and graphene oxide from kusambi wood (Schleichera oleosa)
AU - Adu, Apris A.
AU - Neolaka, Yantus A.B.
AU - Riwu, Arsel A.Pau
AU - Iqbal, Munawar
AU - Darmokoesoemo, Handoko
AU - Kusuma, Heri Septya
N1 - Publisher Copyright:
© 2020 The Author(s).
PY - 2020
Y1 - 2020
N2 - The p53 protein plays an important role by acting as a transcription factor to regulate geneexpression and its genetic mutation has been reported as the most common genetic alter-ations in human cancer diseases. This opens possibilities for the development of earlyidentification of cancer by utilizing p53 protein as tumor biomarkers. In this work wereport the synthesis, characterization and evaluation of p53-poly(MAA-co-EGDMA)@GO-Fe3O4as SPE materials for selective extraction of wild p53 protein. The magnetic materialp53-poly(MAA-co-EGDMA)@GO-Fe3O4(MIP@GO-Fe3O4) is successfully prepared with GO-Fe3O4from kusambi wood (Schleichera oleosa) as the host material, p53 as a templatemolecule, methacrylic acid (MAA) as a monomer, ethylene glycol dimethacrylate (EGDMA)as a crosslinker, benzoyl peroxide (BPO) as an initiator and acetone/ethanol as a porogen.Synthesis is carried out using a bulk polymerization method. To find out the best ratiothat produces the highest MIP@GO-Fe3O4mass, the five variations of the volume ratio oftemplate, monomer and crosslinker (P1-P5) are investigated. It was found that the vol-ume ratio of template, monomer and crosslinker that produced the highest MIP@GO-Fe3O4mass is obtained at P3 volume ratio (1:4:8 mL, which is further polymerized with GO-Fe3O4as much as 0.6239 g). Swelling ratio studies using 3 (three) solvents namely chloroform,acetone and methanol show that the swelling ratio values obtained are 1.46, 1.24 and 1.23 g/g, respectively. These results indicate that methanol is the best solvent for use in the rebind- ing process of MIP@GO-Fe3O4 material. Based on the analysis by FTIR, XRD, SEM-EDX and BET-BJH, the resulting MIP@GO-Fe3O4 material is expected to be used later for wild p53 preconcentration from various biological samples.
AB - The p53 protein plays an important role by acting as a transcription factor to regulate geneexpression and its genetic mutation has been reported as the most common genetic alter-ations in human cancer diseases. This opens possibilities for the development of earlyidentification of cancer by utilizing p53 protein as tumor biomarkers. In this work wereport the synthesis, characterization and evaluation of p53-poly(MAA-co-EGDMA)@GO-Fe3O4as SPE materials for selective extraction of wild p53 protein. The magnetic materialp53-poly(MAA-co-EGDMA)@GO-Fe3O4(MIP@GO-Fe3O4) is successfully prepared with GO-Fe3O4from kusambi wood (Schleichera oleosa) as the host material, p53 as a templatemolecule, methacrylic acid (MAA) as a monomer, ethylene glycol dimethacrylate (EGDMA)as a crosslinker, benzoyl peroxide (BPO) as an initiator and acetone/ethanol as a porogen.Synthesis is carried out using a bulk polymerization method. To find out the best ratiothat produces the highest MIP@GO-Fe3O4mass, the five variations of the volume ratio oftemplate, monomer and crosslinker (P1-P5) are investigated. It was found that the vol-ume ratio of template, monomer and crosslinker that produced the highest MIP@GO-Fe3O4mass is obtained at P3 volume ratio (1:4:8 mL, which is further polymerized with GO-Fe3O4as much as 0.6239 g). Swelling ratio studies using 3 (three) solvents namely chloroform,acetone and methanol show that the swelling ratio values obtained are 1.46, 1.24 and 1.23 g/g, respectively. These results indicate that methanol is the best solvent for use in the rebind- ing process of MIP@GO-Fe3O4 material. Based on the analysis by FTIR, XRD, SEM-EDX and BET-BJH, the resulting MIP@GO-Fe3O4 material is expected to be used later for wild p53 preconcentration from various biological samples.
KW - Bulk polymerization
KW - Graphene oxide
KW - Imprinting polymer
KW - Schleichera oleosa
KW - Wild p53 protein
UR - http://www.scopus.com/inward/record.url?scp=85090445147&partnerID=8YFLogxK
U2 - 10.1016/j.jmrt.2020.08.003
DO - 10.1016/j.jmrt.2020.08.003
M3 - Article
AN - SCOPUS:85090445147
SN - 2238-7854
VL - 9
SP - 11060
EP - 11068
JO - Journal of Materials Research and Technology
JF - Journal of Materials Research and Technology
IS - 5
ER -