TY - GEN
T1 - Synthesis and Characterization of Folic Acid-Functionalized, Doxorubicin-Loaded Magnetic Nanoparticles as Drug Delivery System for Cancer
AU - Widiyanti, Prihartini
AU - Theja, Maria L.V.
AU - Zaidan, Andi H.
N1 - Publisher Copyright:
© 2023 American Institute of Physics Inc.. All rights reserved.
PY - 2023/5/19
Y1 - 2023/5/19
N2 - Cancer is one of the primary causes of death in the world and the prevalence in Indonesia reaches 1,4% per year. One method of the most developed cancer therapies right now is targeting drug delivery. Targeting drug delivery makes it possible for drugs to be delivered directly to the targeted cells and released once they reach the destination. The aim of this research is to develop a nanocarrier using folic acid-functionalized magnetic nanoparticles to improve the effectiveness of antitumor drugs for cancer therapy. The magnetic nanoparticles synthesis method used in this research is chemical synthesis. Chemical materials FeCl3 and FeCl2 are mixed with NH4OH until magnetic nanoparticles appear. The magnetic nanoparticles obtained are then coated using bovine serum albumin, loaded with the drug doxorubicin, and functionalized with folic acid. The characterization methods used are Brine Shrimp Lethal Test (BSLT), X-Ray Diffractometer (XRD), Particle Size Analyzer (PSA), and MTT Assay. The success of drug release test is performed using UV-Vis. The results of this research showed that the magnetic nanoparticles were non-toxic with no impurities, but had not passed the size requirement as a good nanocarrier. Drug release in acidic pH, which is 5.4, had the highest release rate, thus it would be suitable for cancer cells which have lower pH compared to normal cells. Cytotoxicity test using MTT Assay method with murine osteoblastic cell line MC3T3 cells showed quite high cell viability, so it will not affect normal tissues and cells. It can be concluded that folic acid-functionalized, doxorubicin-loaded magnetic nanoparticles have potential to be used as nanocarrier in drug delivery system.
AB - Cancer is one of the primary causes of death in the world and the prevalence in Indonesia reaches 1,4% per year. One method of the most developed cancer therapies right now is targeting drug delivery. Targeting drug delivery makes it possible for drugs to be delivered directly to the targeted cells and released once they reach the destination. The aim of this research is to develop a nanocarrier using folic acid-functionalized magnetic nanoparticles to improve the effectiveness of antitumor drugs for cancer therapy. The magnetic nanoparticles synthesis method used in this research is chemical synthesis. Chemical materials FeCl3 and FeCl2 are mixed with NH4OH until magnetic nanoparticles appear. The magnetic nanoparticles obtained are then coated using bovine serum albumin, loaded with the drug doxorubicin, and functionalized with folic acid. The characterization methods used are Brine Shrimp Lethal Test (BSLT), X-Ray Diffractometer (XRD), Particle Size Analyzer (PSA), and MTT Assay. The success of drug release test is performed using UV-Vis. The results of this research showed that the magnetic nanoparticles were non-toxic with no impurities, but had not passed the size requirement as a good nanocarrier. Drug release in acidic pH, which is 5.4, had the highest release rate, thus it would be suitable for cancer cells which have lower pH compared to normal cells. Cytotoxicity test using MTT Assay method with murine osteoblastic cell line MC3T3 cells showed quite high cell viability, so it will not affect normal tissues and cells. It can be concluded that folic acid-functionalized, doxorubicin-loaded magnetic nanoparticles have potential to be used as nanocarrier in drug delivery system.
KW - bovine serum albumin
KW - cancer
KW - doxorubicin
KW - folic acid
KW - nanoparticles
UR - http://www.scopus.com/inward/record.url?scp=85161365653&partnerID=8YFLogxK
U2 - 10.1063/5.0118718
DO - 10.1063/5.0118718
M3 - Conference contribution
AN - SCOPUS:85161365653
T3 - AIP Conference Proceedings
BT - Proceedings of the International Conference on Advanced Technology and Multidiscipline, ICATAM 2021
A2 - Widiyanti, Prihartini
A2 - Jiwanti, Prastika Krisma
A2 - Prihandana, Gunawan Setia
A2 - Ningrum, Ratih Ardiati
A2 - Prastio, Rizki Putra
A2 - Setiadi, Herlambang
A2 - Rizki, Intan Nurul
PB - American Institute of Physics Inc.
T2 - 1st International Conference on Advanced Technology and Multidiscipline: Advanced Technology and Multidisciplinary Prospective Towards Bright Future, ICATAM 2021
Y2 - 13 October 2021 through 14 October 2021
ER -