Synthesis and Characterization of Collagen-Chitosan-Sodium Hyaluronates as Artificial Cornea

P. Widiyanti, N. D.W. Rini, M. N. Kirana, T. Astutik

Research output: Contribution to journalConference articlepeer-review

2 Citations (Scopus)


As reported by Riset Kesehatan Dasar, Indonesia facing a serious problem of blindness with 966.326 cases of 2 million population in 2013. Gold standard such as keratoplasty couldn't completely heal corneal injury due to the recipient's rejection and limited donor. In order to overcome those concerns, artificial corneal tissue needs to be developed to produce an artificial cornea which is biocompatible, supports corneal function, and applicable. This research was conducted to provide an initial description of Collagen-Chitosan-Natrium Hyaluronate (Col-Chi-NaHA) as an artificial cornea through FTIR analysis, hydrophilicity and water absorption measurement. The artificial cornea was synthesized by forming ratio of collagen 20% w/v and chitosan 10% w/v, both solutions were dissolved in 0,1 M and 0,6 M acetic acid separately. Into the mixed solution, NaHA was dripped slowly with variation of 0%, 0,3%, and 0,6%. The homogenous solution was cast and heated for 24 hours at 35°C. The dry membranes were neutralized to obtain a pH around 7. From the analysis known that all of the characteristic bands of Col-Chi-NaHA could be observed in obtained membranes. Water absorption measurement demonstrated percentages over 90% on all membranes. The contact angle of obtained membranes was decreased by the addition of NaHA. The results showed that artificial cornea made from Col-Chi-NaHA successfully fabricated has hydrophilic properties to support cellular interaction and provide hydration to keep eye's clarity and permeability. Therefore, Col-Chi-NaHA could may be a suitable material for the tissue-engineered cornea.

Original languageEnglish
Article number012035
JournalJournal of Physics: Conference Series
Issue number1
Publication statusPublished - 20 Dec 2019
EventMathematics, Informatics, Science and Education International Conference 2019, MISEIC 2019 - Surabaya, Indonesia
Duration: 28 Sept 2019 → …


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