Supplementation of alpha lipoic acid on Kacang goat (Capra hircus) oocyte to the growth of GDF-9 and BMP-15

Laily ‘U N. ‘ilmi, Tita D. Lestari, Widjiati, Erma Safitri, Tjuk I. Restiadi, Maslichah Mafruchati, Muhammad F. Amrullah, Aswin R. Khairullah, Shendy C. Kurniawan, Salsabilla Abani, Abdullah Hasib, Muhammad E.E. Samodra, Otto S.M. Silaen

Research output: Contribution to journalArticlepeer-review


The application of assisted reproductive technology by mass-producing embryos is to maintain genetic resistance, increase productivity and livestock populations in a short time with maximum quality. Oocyte In vitro Maturation (IVM) is a crucial stage of In vitro Fertilization (IVF) implementation to increase the quantity and quality of livestock genetic trait through in In vitro Embryo Production (IVP). GDF-9 and BMP-15 expression are in the family of Transforming Growth Factor Beta (TGF-β) which are known to regulate oocyte and cumulus cell development in mammals. This study’s objective was to investigate the effects of Alpha Lipoic Acid (ALA) on IVM media of Kacang goat (Capra hircus) oocytes. This research was an experimental study design consist-ing of 3 treatment groups: (P0) the control group, (P1) 25 µM/L ALA supplementation and (P2) 50 µM/L ALA supplementation. This study used 261 COCs which were 22 hours matured in vitro at 38.5°C, 98% humidity, and 5% CO2. GDF-9 and BMP-15 expression data were statistically tested with a significance of p<0.05. Immunocy-tochemistry showed the expression value of GDF-9 (2.86±1.06, 4.29±1.79 and 8.29±2.69). BMP-15 expression values (2.57±1.39, 5.71±3.72 and 11.43±1.51). The results of the study showed that supplementation with ALA on maturation media at a dose of 50 µM/L toward the maturation level and increased GDF-9 and BMP-15 expression in vitro maturation of Kacang goat oocyte.

Original languageEnglish
Pages (from-to)135-138
Number of pages4
JournalJournal of Advanced Veterinary Research
Issue number1
Publication statusPublished - Jan 2024


  • Alpha lipoic acid
  • BMP-15
  • GDF-9
  • In vitro maturation
  • Reproductive healthcare


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