Introduction: Sudamala (Artemisia vulgaris L.) is commonly used in the community as anti-tumor in digestive organ, including in oral cavity. However, there have been no scientific studies, while oral anti-carcinogenic active substances have not been found. The purpose of this study was to explain the effect of per oral administration of n-hexana: ethyl acetate (3:7) fraction of Artemisia vulgaris L. on the reduction of oncogenesis in transformed oral mucosa cells in mice due to benzopyrene induction. Materials and Methods: The experimental laboratory was performed on this study. We used 30 male Mus musculus mice and were divided into 5 groups. Group 1, control, received benzopyrene solvent Oleum Olivarum for 4 weeks, continued with 0.5 % CMC- Na fraction solvent. Group 2 received benzopyrene, followed with 0.5 % CMC-Na. Group 3 also received benzopyrene, followed with 50 mg/ kgbw fraction of Sudamala (Artemisia vulgaris L.). Group 4 received benzopyrene, followed with 100 mg/kgbw fraction. Group 5 received benzopyrene, followed with 200 mg/kgbw fraction of Sudamala (Artemisia vulgaris L.). Tunnel assay staining and immunohistochemistry were undertaken for Ras, P53, PCNA and C-myc. Data were analyzed using Manova test. Results: There is an increase of apoptosis and P53 wild type expression and the reduction of the expression of P53 mutant, Ras, PCNA and C-myc to squamous cells of mice oral cavity which transformed caused by benzopyrene induced due to Artemisia vulgaris L. Conclusions: The fractions of n-hexana: ethyl acetate (3:7) is capable in killing cells which transformed without damaging the healthy ones.

Original languageEnglish
Pages (from-to)79-85
Number of pages7
JournalMalaysian Journal of Medicine and Health Sciences
Publication statusPublished - Feb 2023


  • Benzopyrene
  • Cancer cell
  • Oral cancer
  • Sudamala


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