Study on characterization, pathogenicity and histopathology of disease caused by Aeromonas hydrophila in gourami (Osphronemus gouramy)

Rozi, K. Rahayu, D. N. Daruti, M. S.P. Stella

Research output: Contribution to journalConference articlepeer-review

18 Citations (Scopus)

Abstract

This study aims to determine the bacterial pathogens that cause disease of the gourami in Blitar (East Java) and Yogyakarta (Central Java), Indonesia. A total of 50 fish samples taken randomly gourami in pond farmers in seventh different locations. There were 18 isolates were isolated and then test Koch's postulates were injected 0.1 ml/fish intraperitoneally to gourami. Characterization is done by using the biochemical tests. Pathogenicity test carried out on 3 isolates of Aeromonas spp. with intraperitoneal injection at a dose of 104-108 CFU/fish, the value of Lethal Dosage 50 (LD50) using the method Dragstedt Behrens. After the treatment, spleen and kidney samples were processed for histopathological analysis. The all of identified bacteria were 5 isolates Aeromonas hydrophila. Isolates of A. hydrophila in a row AH3 was virulen to gourami with LD50 (4.53 x 106 CFU/fish), while isolate AH4 and AH5 (2.903 x 108, 1.319 x 109 CFU/fish) not be avirulen. Koch's postulates; 3 isolates are pathogenic with mortality of 40-100 % and 2 are non-pathogenic isolates with a mortality of 0 %. Clinically; ulcers, haemorhagic at the base of the fins, body, mouth and exophthalmia. Histopathologically indecated spleen necrosis, piknosis, necrosis and inflammatory cells in kidney.

Original languageEnglish
Article number012003
JournalIOP Conference Series: Earth and Environmental Science
Volume137
Issue number1
DOIs
Publication statusPublished - 13 Apr 2018
Event7th ASEAN-FEN International Fisheries Symposium, IFS 2017 - Batu City, East Java, Indonesia
Duration: 7 Nov 20179 Nov 2017

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