TY - GEN
T1 - Study of Levofloxacin Electrochemical Detection on Nickel Electrode
AU - Jiwanti, Prastika Krisma
AU - Sitorus, Irfansyah Rais
N1 - Publisher Copyright:
© 2023 American Institute of Physics Inc.. All rights reserved.
PY - 2023/5/19
Y1 - 2023/5/19
N2 - Levofloxacin (LEV) is one of fluoroquinolone type of antibiotic. Aside from its effectiveness to treat infectious diseases, LEV may cause some severe heart problem and also bacterial resistance. Therefore, the determination of the LEV antibiotic in biological samples (urine or human blood) is necessary. In this report, electrochemical method was use for LEV analysis on nickel (Ni) electrode as working electrode, platinum as counter electrode, and Ag/AgCl as reference electrode. Cyclic voltammetry (CV) and square wave voltammetry (SWV) were used for electroanalytical study. The SWV condition was optimized. Optimum amplitude, frequency, and step potential were 50 mV, 50 Hz, 0.012 mV respectively. The LEV analysis was also optimized by varying the pH, in which, optimum current was observed at pH 5.5. The detection of LEV was observed by analyzing the peak oxidation of LEV, that was clearly observed at potential around +1.0 V with S/B of 3.5. From the calibration curved of LEV, the limit of detection (LOD) and limit of quantification (LOQ) of LEV were calculated and the value of 11.91 µM and 39,71 µM respectively was obtained. The results also show the satisfactory of reproducibility with relative standard deviation (RSD) of 2.93%. Finally, this sensor is stable and repeatable, feasible for direct analysis to the real determination of LEV in biological sample.
AB - Levofloxacin (LEV) is one of fluoroquinolone type of antibiotic. Aside from its effectiveness to treat infectious diseases, LEV may cause some severe heart problem and also bacterial resistance. Therefore, the determination of the LEV antibiotic in biological samples (urine or human blood) is necessary. In this report, electrochemical method was use for LEV analysis on nickel (Ni) electrode as working electrode, platinum as counter electrode, and Ag/AgCl as reference electrode. Cyclic voltammetry (CV) and square wave voltammetry (SWV) were used for electroanalytical study. The SWV condition was optimized. Optimum amplitude, frequency, and step potential were 50 mV, 50 Hz, 0.012 mV respectively. The LEV analysis was also optimized by varying the pH, in which, optimum current was observed at pH 5.5. The detection of LEV was observed by analyzing the peak oxidation of LEV, that was clearly observed at potential around +1.0 V with S/B of 3.5. From the calibration curved of LEV, the limit of detection (LOD) and limit of quantification (LOQ) of LEV were calculated and the value of 11.91 µM and 39,71 µM respectively was obtained. The results also show the satisfactory of reproducibility with relative standard deviation (RSD) of 2.93%. Finally, this sensor is stable and repeatable, feasible for direct analysis to the real determination of LEV in biological sample.
UR - http://www.scopus.com/inward/record.url?scp=85161398652&partnerID=8YFLogxK
U2 - 10.1063/5.0119160
DO - 10.1063/5.0119160
M3 - Conference contribution
AN - SCOPUS:85161398652
T3 - AIP Conference Proceedings
BT - Proceedings of the International Conference on Advanced Technology and Multidiscipline, ICATAM 2021
A2 - Widiyanti, Prihartini
A2 - Jiwanti, Prastika Krisma
A2 - Prihandana, Gunawan Setia
A2 - Ningrum, Ratih Ardiati
A2 - Prastio, Rizki Putra
A2 - Setiadi, Herlambang
A2 - Rizki, Intan Nurul
PB - American Institute of Physics Inc.
T2 - 1st International Conference on Advanced Technology and Multidiscipline: Advanced Technology and Multidisciplinary Prospective Towards Bright Future, ICATAM 2021
Y2 - 13 October 2021 through 14 October 2021
ER -
