TY - JOUR
T1 - Study of Cellulase Activity Produced by Penicillium sp., Aspergillus niger and Trichoderma viride on Imperata cylindrica (L.) Beauv. Enrichment Media
AU - Agustina, D.
AU - Surtiningsih, T.
AU - Manuhara, Y. S.W.
AU - Arifiyanto, A.
AU - Malewa, M.
N1 - Publisher Copyright:
© 2019 Published under licence by IOP Publishing Ltd.
PY - 2019/4/9
Y1 - 2019/4/9
N2 - The aim of this study was to determine the value of cellulase activity produced by mould toward filter paperase (FP-ase) and endoglucanase (EG). Penicillium sp., Aspergillus niger and Trichoderma viride utilized as mould isolate. Cogon grass substrates pre-treated with 2% NaOH. It determined the reducing sugar. The respective cellulase activity of each isolate was measured using filter paperase (FP-ase) and endoglucanase (EG). Pre-treatment successfully damaged lignin structure. The most favourable incubation period produced in 7th days. Moreover, it increased surface area of cellulose with a cellulose level of 48.32%, hemicellulose level of 9.88% and lignin level of 1.14%. Aspergillus niger exhibit the highest cellulase activity with enzymes value of 3.97 U/ml for filter paperase (FP-ase) and 4.53 U/ml for endoglucanase (EG). Penicillium sp. reached 3.71 U/ml for filter paperase (FP-ase) and 4.11 U/ml for endoglucanase (EG) while Trichoderma viride got 3.37 U/ml for filter paperase (FP-ase) and 3.38 U/ml for endoglucanase (EG).
AB - The aim of this study was to determine the value of cellulase activity produced by mould toward filter paperase (FP-ase) and endoglucanase (EG). Penicillium sp., Aspergillus niger and Trichoderma viride utilized as mould isolate. Cogon grass substrates pre-treated with 2% NaOH. It determined the reducing sugar. The respective cellulase activity of each isolate was measured using filter paperase (FP-ase) and endoglucanase (EG). Pre-treatment successfully damaged lignin structure. The most favourable incubation period produced in 7th days. Moreover, it increased surface area of cellulose with a cellulose level of 48.32%, hemicellulose level of 9.88% and lignin level of 1.14%. Aspergillus niger exhibit the highest cellulase activity with enzymes value of 3.97 U/ml for filter paperase (FP-ase) and 4.53 U/ml for endoglucanase (EG). Penicillium sp. reached 3.71 U/ml for filter paperase (FP-ase) and 4.11 U/ml for endoglucanase (EG) while Trichoderma viride got 3.37 U/ml for filter paperase (FP-ase) and 3.38 U/ml for endoglucanase (EG).
UR - http://www.scopus.com/inward/record.url?scp=85064861415&partnerID=8YFLogxK
U2 - 10.1088/1755-1315/253/1/012016
DO - 10.1088/1755-1315/253/1/012016
M3 - Conference article
AN - SCOPUS:85064861415
SN - 1755-1307
VL - 253
JO - IOP Conference Series: Earth and Environmental Science
JF - IOP Conference Series: Earth and Environmental Science
IS - 1
M1 - 012016
T2 - 1st MarSave International Symposium 2018: Strengthening Marine Resilience for Sustainable Development Goals
Y2 - 7 August 2018 through 8 August 2018
ER -