STUDY OF ANTIBACTERIAL ABILITY OF HYLOCEREUS POLYRHIZUS FOR GRAM NEGATIVE BACTERIA

Gram-negative bacteria are bacteria that have a cell wall layer <10 nm and have an outer lipid bilayer in the form of lipopolysaccharide (LPS). Fusobacterium nucleaum, Aggregatibacter actinomycetemcomitans and Porphyromonas gingivalis are gram-negative bacteria, which are one of the main causes of periodontitis. Red dragon fruit skin contains several antibacterial compounds in the form of flavonoids and terpenoids. Previous research has been conducted on the antibacterial ability of red dragon fruit peel extract against several gram-negative bacteria, but the antibacterial ability of red dragon fruit peel extract against F. nucleaum, A. actinomycetemcomitans and P. gingivalis has not been tested. This study goal was to assesing the antibacterial ability of red dragon fruit slom extract against F. nucleaum, A. actinomycetemcomitans and P. gingivalis through Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) tests. MIC measurement of red dragon fruit extract against P. gingivalis, A. actinomycetemcomitans and F. nucleatum was carried out by serial dilution method on BHI media. Determination of MBC is done by taking a sample of bacteria from the liquid media, which is used for MIC measurements into the agar medium. The MIC and MBC values of P. gingivalis, A. actinomycetemcomitans and F. nucleatum were 6.25% and 12.5%. Red dragon fruit skin extract has the ability to inhibit the growth of P. gingivalis, A. actinomycetemcomitans and F. nucleatum.