Stability of (-)- Epigallocatechin Gallate (EGCG) in Green Tea Instant Beverage Product Using High Performance Liquid Chromatography (HPLC) Method

Fahmi Dimas Abdul Azis, Hamidah Nurlaila, Djoko Agus Purwanto, Ahmad Taufiq

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

1 Citation (Scopus)

Abstract

(-)Epigallocatechin gallate (EGCG), the higest phytochemical compound contained from tea leaf (Camellia sinensis), has been proven to improve blood flow, prevent cardiovascular disease, and eliminate various toxins, improving resistance to various diseases, by its anti-oxidant activity. Green tea catechins especially EGCG can undergo degradation and epimerization during storage conditions. Many factors could contribute to the stability of green tea catechins, including EGCG, such as temperature and duration of storage. The research focused on studying the duration stability of EGCG during storage in low temperature (8°C) using HPLC. HPLC conditions were as follows: C-18 reversed-phase μbondapak 10 urn, 3.9 × 300 mm used as the column, the mobile phase was methanol: water: acetic acid (2%) (30: 65: 5 v/v/v), the flow rate of 1.0 ml/min, and detection monitored at 276.0 nm with spectrophotometry UV-Vis. The levels of EGCG during 32 hours of storage using the refrigerator in 8°C decreased by 20.11%, and the levels of EGCG in 25°C decreased by 26,73%. It was proven that there were significant differences between the level decrease of EGCG in green tea instant beverage products in the refrigerator (8°C) and storage at 25°C.

Original languageEnglish
Title of host publication4th International Conference on Life Science and Technology, ICoLiST 2023
EditorsAhmad Taufiq, Hendra Susanto, I. Ching Wang, Hadi Nur, Muhammad Aziz, Chuang-Rung Chang, Gereon Elbers, Sri Rahayu Lestari, Markus Diantoro, Nandang Mufti, Chen Siang Ng, Nik Ahmad Nizam Nik Malek, Sunaryono, Yu-Hsin Chiu, Siti Zubaidah, Aulanni'am, Indra Wibowo, Adeodatus Yuda Handaya
PublisherAmerican Institute of Physics Inc.
ISBN (Electronic)9780735443136
DOIs
Publication statusPublished - 24 Jan 2023
Event4th International Conference on Life Science and Technology, ICoLiST 2023 - Virtual, Online, Indonesia
Duration: 31 Aug 2021 → …

Publication series

NameAIP Conference Proceedings
Volume2634
ISSN (Print)0094-243X
ISSN (Electronic)1551-7616

Conference

Conference4th International Conference on Life Science and Technology, ICoLiST 2023
Country/TerritoryIndonesia
CityVirtual, Online
Period31/08/21 → …

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