TY - GEN
T1 - Sperm Na + ,K + -ATPase and dynein ATPase activity
T2 - 3rd International Symposium of Biomedical Engineering''s Recent Progress in Biomaterials, Drugs Development, and Medical Devices, ISBE 2018
AU - Lestari, Silvia W.
AU - Hinting, Aucky
AU - Lunardi, Hamdani
AU - Aditya, Debby
AU - Miati, Dessy Noor
AU - Rizki, Meidika Dara
N1 - Publisher Copyright:
© 2019 Author(s).
PY - 2019/4/9
Y1 - 2019/4/9
N2 - The published data represented that poor semen parameters resulted in the poor embryo development rate following in vitro Fertilization (IVF) - intracytoplasmic sperm injection (ICSI). The disorder of sperm motility involving Na + ,K + -ATPase and dynein ATPase enzymes disruption may contribute to the paternal effects on embryo quality and development. This study investigated the activities of Na + ,K + -ATPase and dynein ATPase in human sperms of infertile men and the correlation to the ICSI outcome referring to the embryo development. Semen samples were obtained from men performing sperm preparation for the ICSI. The World Health Organization (WHO) 2010 Guidance was used to perform semen analysis. Next, the enzyme activity was measured based on the ability of ATPase to release organic phosphate from ATP as a substrate. The ICSI outcomes including the embryo development grading and scoring were recorded and analyzed. The value of Na + ,K + -ATPase and dynein ATPase were 1.847±0.694 and 1.459±0.843μmol Pi/mg protein/h, while the embryo scoring was 91.83. Using the cut-off value of Na + ,K + -ATPase and dynein ATPase, we found that the low level Na + ,K + -ATPase and dynein ATPase correlated with the low embryo development quality (p<0.05 and p>0.05, respectively). This study demonstrated that poor activities of Na + ,K + -ATPase and dynein ATPase could affect embryo the development following the ICSI.
AB - The published data represented that poor semen parameters resulted in the poor embryo development rate following in vitro Fertilization (IVF) - intracytoplasmic sperm injection (ICSI). The disorder of sperm motility involving Na + ,K + -ATPase and dynein ATPase enzymes disruption may contribute to the paternal effects on embryo quality and development. This study investigated the activities of Na + ,K + -ATPase and dynein ATPase in human sperms of infertile men and the correlation to the ICSI outcome referring to the embryo development. Semen samples were obtained from men performing sperm preparation for the ICSI. The World Health Organization (WHO) 2010 Guidance was used to perform semen analysis. Next, the enzyme activity was measured based on the ability of ATPase to release organic phosphate from ATP as a substrate. The ICSI outcomes including the embryo development grading and scoring were recorded and analyzed. The value of Na + ,K + -ATPase and dynein ATPase were 1.847±0.694 and 1.459±0.843μmol Pi/mg protein/h, while the embryo scoring was 91.83. Using the cut-off value of Na + ,K + -ATPase and dynein ATPase, we found that the low level Na + ,K + -ATPase and dynein ATPase correlated with the low embryo development quality (p<0.05 and p>0.05, respectively). This study demonstrated that poor activities of Na + ,K + -ATPase and dynein ATPase could affect embryo the development following the ICSI.
KW - Na ,K ATPase
KW - dynein ATPase
KW - embryo development quality
UR - http://www.scopus.com/inward/record.url?scp=85064822354&partnerID=8YFLogxK
U2 - 10.1063/1.5096752
DO - 10.1063/1.5096752
M3 - Conference contribution
AN - SCOPUS:85064822354
T3 - AIP Conference Proceedings
BT - 3rd Biomedical Engineering''s Recent Progress in Biomaterials, Drugs Development, and Medical Devices
A2 - Wulan, Praswasti P.D.K.
A2 - Gozan, Misri
A2 - Astutiningsih, Sotya
A2 - Ramahdita, Ghiska
A2 - Dhelika, Radon
A2 - Kreshanti, Prasetyanugraheni
PB - American Institute of Physics Inc.
Y2 - 6 August 2018 through 8 August 2018
ER -
