The published data represented that poor semen parameters resulted in the poor embryo development rate following in vitro Fertilization (IVF) - intracytoplasmic sperm injection (ICSI). The disorder of sperm motility involving Na + ,K + -ATPase and dynein ATPase enzymes disruption may contribute to the paternal effects on embryo quality and development. This study investigated the activities of Na + ,K + -ATPase and dynein ATPase in human sperms of infertile men and the correlation to the ICSI outcome referring to the embryo development. Semen samples were obtained from men performing sperm preparation for the ICSI. The World Health Organization (WHO) 2010 Guidance was used to perform semen analysis. Next, the enzyme activity was measured based on the ability of ATPase to release organic phosphate from ATP as a substrate. The ICSI outcomes including the embryo development grading and scoring were recorded and analyzed. The value of Na + ,K + -ATPase and dynein ATPase were 1.847±0.694 and 1.459±0.843μmol Pi/mg protein/h, while the embryo scoring was 91.83. Using the cut-off value of Na + ,K + -ATPase and dynein ATPase, we found that the low level Na + ,K + -ATPase and dynein ATPase correlated with the low embryo development quality (p<0.05 and p>0.05, respectively). This study demonstrated that poor activities of Na + ,K + -ATPase and dynein ATPase could affect embryo the development following the ICSI.