TY - JOUR
T1 - Sperm Kinematics and Morphology of Bali Bull (Bos javanicus) after Freezing and Thawing Treated with Green Tea Extract in Extender
AU - Prastiya, R. A.
AU - Debora, A. E.
AU - Wijayanti, A.
AU - Agustono, B.
AU - Saputro, A. L.
AU - Amaliya, A.
AU - Sasi, S. M.
N1 - Publisher Copyright:
© 2023 by Authors, published by Tropical Animal Science Journal. This is an open-access article distributed under the CC BY-SA 4.0 License (https://creativecommons.Org/licenses/by/4.0/). All Rights Reserved.
PY - 2023
Y1 - 2023
N2 - Green Tea Extract, or GTE, is a powerful antioxidant that can be added to the extender to maintain the quality of Bali bull semen before and after freezing. This study aimed to evaluate the impact of the GTE addition in the extender on the sperm kinematic and sperm morphology from frozen semen of Bali bull. Five Bali bulls from the NAIC, were used as semen sources. In this study, there were four different treatments: a control (Tris-egg yolk without GTE), a GTE0.05 (100 mL tris egg yolk with 0.05 mg of GTE), a GTE0.10 (100 mL tris egg yolk with 0.10 mg of GTE), and a GTE0.15 (100 mL tris egg yolk with 0.15 mg of GTE). A sperm analyzer (CASA) was used to observe the sperm kinematics and morphology. Compared to the control group, adding different concentrations of GTE to the semen extender did not affect the sperm’s morphology. Before freezing, adding GTE0.05 increases total and progressive motility as well as VAP, VCL, and VSL after thawing. GTE0.15 increases STR and ALH. The conclusion is that GTE additions at doses of 0.05 mg/100 mL and 0.15 mg/100 mL can increase sperm kinematics parameters, but sperm morphology is not affected by the GTE.
AB - Green Tea Extract, or GTE, is a powerful antioxidant that can be added to the extender to maintain the quality of Bali bull semen before and after freezing. This study aimed to evaluate the impact of the GTE addition in the extender on the sperm kinematic and sperm morphology from frozen semen of Bali bull. Five Bali bulls from the NAIC, were used as semen sources. In this study, there were four different treatments: a control (Tris-egg yolk without GTE), a GTE0.05 (100 mL tris egg yolk with 0.05 mg of GTE), a GTE0.10 (100 mL tris egg yolk with 0.10 mg of GTE), and a GTE0.15 (100 mL tris egg yolk with 0.15 mg of GTE). A sperm analyzer (CASA) was used to observe the sperm kinematics and morphology. Compared to the control group, adding different concentrations of GTE to the semen extender did not affect the sperm’s morphology. Before freezing, adding GTE0.05 increases total and progressive motility as well as VAP, VCL, and VSL after thawing. GTE0.15 increases STR and ALH. The conclusion is that GTE additions at doses of 0.05 mg/100 mL and 0.15 mg/100 mL can increase sperm kinematics parameters, but sperm morphology is not affected by the GTE.
KW - Bali bull
KW - green tea extract
KW - kinematics
KW - livestock and gene bank
KW - sperm cryopreservation
UR - http://www.scopus.com/inward/record.url?scp=85182251047&partnerID=8YFLogxK
U2 - 10.5398/TASJ.2023.46.4.418
DO - 10.5398/TASJ.2023.46.4.418
M3 - Article
AN - SCOPUS:85182251047
SN - 2615-787X
VL - 46
SP - 418
EP - 427
JO - Tropical Animal Science Journal
JF - Tropical Animal Science Journal
IS - 4
ER -