TY - JOUR
T1 - Species diversity of Entamoeba and gastrointestinal parasites as co-infection in pigs in Kupang, East Nusa Tenggara, Indonesia
AU - Chrismanto, Dony
AU - Lastuti, Nunuk Dyah Retno
AU - Suwanti, Lucia Tri
AU - Hastutiek, Poedji
AU - Suprihati, Endang
AU - Kurniawati, Dyah Ayu
AU - Winarso, Aji
N1 - Publisher Copyright:
© 2023, Society for Indonesian Biodiversity. All rights reserved.
PY - 2023
Y1 - 2023
N2 - Protozoa Entamoeba spp. is the causative agent of amoebiasis that can infect humans and livestock. Several species that infect pigs are E. suis, E. polecki, E. histolytica, and E. coli. However, microscopic examination through morphological identification of parasites couldn't determine the genus and species level identity, leading to misdiagnosis. Therefore, molecular techniques are needed for accurate diagnosis with species-specific primers. In this study, 100 fecal samples were collected from pig farms in Kupang, East Nusa Tenggara Province, Indonesia. This study aims to identify, using molecular techniques, the presence of Entamoeba spp. with the SSUrRNA gene as a marker to determine the diversity of Entamoeba species in pigs and to detect co-infection with other gastrointestinal parasites in Kupang. The result was found: E. suis 98% (98/100), E. polecki 44% (44/100), E. polecki ST3 10% (10/100), and E. coli 20% (30/100) spread on pig farms in 8 villages in Kupang. Furthermore, E. polecki ST3 and E. coli finding show that pigs in several areas in Kupang are infected with potentially zoonotic species. In addition to finding various species of Entamoeba by Polymerase Chain Reaction (PCR) amplification, based on microscopic examination, other gastrointestinal parasites were also found as co-infections, namely: Eimeria sp. (34%), Cystoisospora suis (30%), Strongyloides sp. (34%), Oesophagostomum sp. (51%), Metastrongylus sp. (18%), and Trichuris suis (8%). That is the first report concerning mixed infections with E. suis, E. polecki ST3, E. coli, and other gastrointestinal parasites in pigs in Kupang, Indonesia.
AB - Protozoa Entamoeba spp. is the causative agent of amoebiasis that can infect humans and livestock. Several species that infect pigs are E. suis, E. polecki, E. histolytica, and E. coli. However, microscopic examination through morphological identification of parasites couldn't determine the genus and species level identity, leading to misdiagnosis. Therefore, molecular techniques are needed for accurate diagnosis with species-specific primers. In this study, 100 fecal samples were collected from pig farms in Kupang, East Nusa Tenggara Province, Indonesia. This study aims to identify, using molecular techniques, the presence of Entamoeba spp. with the SSUrRNA gene as a marker to determine the diversity of Entamoeba species in pigs and to detect co-infection with other gastrointestinal parasites in Kupang. The result was found: E. suis 98% (98/100), E. polecki 44% (44/100), E. polecki ST3 10% (10/100), and E. coli 20% (30/100) spread on pig farms in 8 villages in Kupang. Furthermore, E. polecki ST3 and E. coli finding show that pigs in several areas in Kupang are infected with potentially zoonotic species. In addition to finding various species of Entamoeba by Polymerase Chain Reaction (PCR) amplification, based on microscopic examination, other gastrointestinal parasites were also found as co-infections, namely: Eimeria sp. (34%), Cystoisospora suis (30%), Strongyloides sp. (34%), Oesophagostomum sp. (51%), Metastrongylus sp. (18%), and Trichuris suis (8%). That is the first report concerning mixed infections with E. suis, E. polecki ST3, E. coli, and other gastrointestinal parasites in pigs in Kupang, Indonesia.
KW - Entamoeba spp.
KW - Gastrointestinal parasites
KW - SSUrRNA
KW - Suis sp.
UR - http://www.scopus.com/inward/record.url?scp=85146565306&partnerID=8YFLogxK
U2 - 10.13057/biodiv/d240128
DO - 10.13057/biodiv/d240128
M3 - Article
AN - SCOPUS:85146565306
SN - 1412-033X
VL - 24
SP - 233
EP - 240
JO - Biodiversitas
JF - Biodiversitas
IS - 1
ER -