Short communication: The presence of extended-spectrum beta-lactamase (ESBL) producing escherichia coli on layer chicken farms in Blitar area, Indonesia

Freshinta Jellia Wibisono; Bambang Sumiarto; Tri Untari; Mustofa Helmi Effendi; Dian Ayu Permatasari; Adiana Mutamsari Witaningrum

doi:10.13057/biodiv/d210638

Short communication: The presence of extended-spectrum beta-lactamase (ESBL) producing escherichia coli on layer chicken farms in Blitar area, Indonesia

Freshinta Jellia Wibisono, Bambang Sumiarto, Tri Untari, Mustofa Helmi Effendi, Dian Ayu Permatasari, Adiana Mutamsari Witaningrum

Research output: Contribution to journal › Article › peer-review

27 Citations (Scopus)

Abstract

This study was aimed to determine the incidence of Extended-Spectrum Beta-Lactamase (ESBL) producing Escherichia coli on layer chicken in Blitar area. This was a cross-sectional study with a total of 205 cloacal swabs of layer chicken taken randomly. The sample was in isolation identification on MacConkey media and ESBL confirmation test produced by Escherichia coli was then carried out by the Double Disc Synergy Test (DDST) method and the VITEK® 2 Compact Automated System method. This study showed that 185 (90.24%) isolates of positive Escherichia coli from a total of 205 samples of cloacal swabs of the layer chicken. The incidence of ESBL-producing Escherichia coli in cloacal swabs on layer chicken with the Double Disc Synergy Test (DDST) method and the VITEK® 2 compact automatic method was 13 (7.03%). Results in this study indicated that layer chicken has potential as reservoir for spreading ESBL to public health and needs strict hygienic measures to prevent their transmission to humans.

Original language	English
Pages (from-to)	2667-2671
Number of pages	5
Journal	Biodiversitas
Volume	21
Issue number	6
DOIs	https://doi.org/10.13057/biodiv/d210638
Publication status	Published - Jun 2020

Keywords

Cloacal swab
DDST
ESBL
Escherichia coli
Layer chicken
VITEK®2

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.13057/biodiv/d210638

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title = "Short communication: The presence of extended-spectrum beta-lactamase (ESBL) producing escherichia coli on layer chicken farms in Blitar area, Indonesia",

abstract = "This study was aimed to determine the incidence of Extended-Spectrum Beta-Lactamase (ESBL) producing Escherichia coli on layer chicken in Blitar area. This was a cross-sectional study with a total of 205 cloacal swabs of layer chicken taken randomly. The sample was in isolation identification on MacConkey media and ESBL confirmation test produced by Escherichia coli was then carried out by the Double Disc Synergy Test (DDST) method and the VITEK{\textregistered} 2 Compact Automated System method. This study showed that 185 (90.24%) isolates of positive Escherichia coli from a total of 205 samples of cloacal swabs of the layer chicken. The incidence of ESBL-producing Escherichia coli in cloacal swabs on layer chicken with the Double Disc Synergy Test (DDST) method and the VITEK{\textregistered} 2 compact automatic method was 13 (7.03%). Results in this study indicated that layer chicken has potential as reservoir for spreading ESBL to public health and needs strict hygienic measures to prevent their transmission to humans.",

keywords = "Cloacal swab, DDST, ESBL, Escherichia coli, Layer chicken, VITEK{\textregistered}2",

author = "Wibisono, {Freshinta Jellia} and Bambang Sumiarto and Tri Untari and Effendi, {Mustofa Helmi} and Permatasari, {Dian Ayu} and Witaningrum, {Adiana Mutamsari}",

note = "Funding Information: We would like to express our heartfelt thanks to Rector of Airlangga University for providing funding for this study by Hibah Mandat Universitas Airlangga. We also would like to thank the Animal Husbandry Services of Blitar District for the collaboration during the recruitment process and provided surveillance data. This study was supported by grants Hibah Mandat with number: 371/UN3.14/LT/2019 from the Universitas Airlangga. Publisher Copyright: {\textcopyright} 2020, Society for Indonesian Biodiversity. All rights reserved.",

year = "2020",

month = jun,

doi = "10.13057/biodiv/d210638",

language = "English",

volume = "21",

pages = "2667--2671",

journal = "Biodiversitas",

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AU - Wibisono, Freshinta Jellia

AU - Sumiarto, Bambang

AU - Untari, Tri

AU - Effendi, Mustofa Helmi

AU - Permatasari, Dian Ayu

AU - Witaningrum, Adiana Mutamsari

N1 - Funding Information: We would like to express our heartfelt thanks to Rector of Airlangga University for providing funding for this study by Hibah Mandat Universitas Airlangga. We also would like to thank the Animal Husbandry Services of Blitar District for the collaboration during the recruitment process and provided surveillance data. This study was supported by grants Hibah Mandat with number: 371/UN3.14/LT/2019 from the Universitas Airlangga. Publisher Copyright: © 2020, Society for Indonesian Biodiversity. All rights reserved.

PY - 2020/6

Y1 - 2020/6

N2 - This study was aimed to determine the incidence of Extended-Spectrum Beta-Lactamase (ESBL) producing Escherichia coli on layer chicken in Blitar area. This was a cross-sectional study with a total of 205 cloacal swabs of layer chicken taken randomly. The sample was in isolation identification on MacConkey media and ESBL confirmation test produced by Escherichia coli was then carried out by the Double Disc Synergy Test (DDST) method and the VITEK® 2 Compact Automated System method. This study showed that 185 (90.24%) isolates of positive Escherichia coli from a total of 205 samples of cloacal swabs of the layer chicken. The incidence of ESBL-producing Escherichia coli in cloacal swabs on layer chicken with the Double Disc Synergy Test (DDST) method and the VITEK® 2 compact automatic method was 13 (7.03%). Results in this study indicated that layer chicken has potential as reservoir for spreading ESBL to public health and needs strict hygienic measures to prevent their transmission to humans.

AB - This study was aimed to determine the incidence of Extended-Spectrum Beta-Lactamase (ESBL) producing Escherichia coli on layer chicken in Blitar area. This was a cross-sectional study with a total of 205 cloacal swabs of layer chicken taken randomly. The sample was in isolation identification on MacConkey media and ESBL confirmation test produced by Escherichia coli was then carried out by the Double Disc Synergy Test (DDST) method and the VITEK® 2 Compact Automated System method. This study showed that 185 (90.24%) isolates of positive Escherichia coli from a total of 205 samples of cloacal swabs of the layer chicken. The incidence of ESBL-producing Escherichia coli in cloacal swabs on layer chicken with the Double Disc Synergy Test (DDST) method and the VITEK® 2 compact automatic method was 13 (7.03%). Results in this study indicated that layer chicken has potential as reservoir for spreading ESBL to public health and needs strict hygienic measures to prevent their transmission to humans.

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ER -

Short communication: The presence of extended-spectrum beta-lactamase (ESBL) producing escherichia coli on layer chicken farms in Blitar area, Indonesia

Abstract

Keywords

UN SDGs

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