TY - GEN
T1 - Sea cucumber species identification of family Caudinidae from Surabaya based on morphological and mitochondrial DNA evidence
AU - Amin, Muhammad Hilman Fu Adil
AU - Pidada, Ida Bagus Rai
AU - Sugiharto,
AU - Widyatmoko, Johan Nuari
AU - Irawan, Bambang
N1 - Publisher Copyright:
© 2016 AIP Publishing LLC.
PY - 2016/3/15
Y1 - 2016/3/15
N2 - Species identification and taxonomy of sea cucumber remains a challenge problem in some taxa. Caudinidae family of sea cucumber was comerciallized in Surabaya, and it was used as sea cucumber chips. Members of Caudinid sea cucumber have similiar morphology, so it is hard to identify this sea cucumber only from morphological appearance. DNA barcoding is useful method to overcome this problem. The aim of this study was to determine Caudinid specimen of sea cucumber in East Java by morphological and molecular approach. Sample was collected from east coast of Surabaya, then preserved in absolute ethanol. After DNA isolation, Cytochrome Oxydase I (COI) gene amplification was performed using Echinoderm universal primer and PCR product was sequenced. Sequencing result was analyzed and identified in NCBI database using BLAST. Results showed that Caudinid specimen in have closely related to Acaudina molpadioides sequence in GenBank with 86% identity. Morphological data, especially based on ossicle, also showed that the specimen is Acaudina molpadioides.
AB - Species identification and taxonomy of sea cucumber remains a challenge problem in some taxa. Caudinidae family of sea cucumber was comerciallized in Surabaya, and it was used as sea cucumber chips. Members of Caudinid sea cucumber have similiar morphology, so it is hard to identify this sea cucumber only from morphological appearance. DNA barcoding is useful method to overcome this problem. The aim of this study was to determine Caudinid specimen of sea cucumber in East Java by morphological and molecular approach. Sample was collected from east coast of Surabaya, then preserved in absolute ethanol. After DNA isolation, Cytochrome Oxydase I (COI) gene amplification was performed using Echinoderm universal primer and PCR product was sequenced. Sequencing result was analyzed and identified in NCBI database using BLAST. Results showed that Caudinid specimen in have closely related to Acaudina molpadioides sequence in GenBank with 86% identity. Morphological data, especially based on ossicle, also showed that the specimen is Acaudina molpadioides.
KW - Caudinidae
KW - Cytochrome Oxydase I
KW - Surabaya
KW - ossicle
UR - http://www.scopus.com/inward/record.url?scp=84984540707&partnerID=8YFLogxK
U2 - 10.1063/1.4943311
DO - 10.1063/1.4943311
M3 - Conference contribution
AN - SCOPUS:84984540707
T3 - AIP Conference Proceedings
BT - 5th International Conference and Workshop on Basic and Applied Sciences, ICOWOBAS 2015
A2 - Yasin, Moh.
A2 - Harun, Sulaiman W.
PB - American Institute of Physics Inc.
T2 - 5th International Conference and Workshop on Basic and Applied Sciences, ICOWOBAS 2015
Y2 - 16 October 2015 through 17 October 2015
ER -