Sea cucumber species identification of family Caudinidae from Surabaya based on morphological and mitochondrial DNA evidence

Muhammad Hilman Fu Adil Amin, Ida Bagus Rai Pidada, Sugiharto, Johan Nuari Widyatmoko, Bambang Irawan

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

11 Citations (Scopus)

Abstract

Species identification and taxonomy of sea cucumber remains a challenge problem in some taxa. Caudinidae family of sea cucumber was comerciallized in Surabaya, and it was used as sea cucumber chips. Members of Caudinid sea cucumber have similiar morphology, so it is hard to identify this sea cucumber only from morphological appearance. DNA barcoding is useful method to overcome this problem. The aim of this study was to determine Caudinid specimen of sea cucumber in East Java by morphological and molecular approach. Sample was collected from east coast of Surabaya, then preserved in absolute ethanol. After DNA isolation, Cytochrome Oxydase I (COI) gene amplification was performed using Echinoderm universal primer and PCR product was sequenced. Sequencing result was analyzed and identified in NCBI database using BLAST. Results showed that Caudinid specimen in have closely related to Acaudina molpadioides sequence in GenBank with 86% identity. Morphological data, especially based on ossicle, also showed that the specimen is Acaudina molpadioides.

Original languageEnglish
Title of host publication5th International Conference and Workshop on Basic and Applied Sciences, ICOWOBAS 2015
EditorsMoh. Yasin, Sulaiman W. Harun
PublisherAmerican Institute of Physics Inc.
ISBN (Electronic)9780735413641
DOIs
Publication statusPublished - 15 Mar 2016
Event5th International Conference and Workshop on Basic and Applied Sciences, ICOWOBAS 2015 - Surabaya, Indonesia
Duration: 16 Oct 201517 Oct 2015

Publication series

NameAIP Conference Proceedings
Volume1718
ISSN (Print)0094-243X
ISSN (Electronic)1551-7616

Conference

Conference5th International Conference and Workshop on Basic and Applied Sciences, ICOWOBAS 2015
Country/TerritoryIndonesia
CitySurabaya
Period16/10/1517/10/15

Keywords

  • Caudinidae
  • Cytochrome Oxydase I
  • Surabaya
  • ossicle

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