Screening the reproductive tract of dairy cattle for pathogenic micros

The study aims to identify bacteria in reproductive tract of Holstein Fresian (HF) dairy cattle, post artificial insemination at KSU Tunas Setia Baru Tutur Sub district, Pasuruan, East Java, Indonesia, which can cause reproductive disorder. Methodology used in the study was bacteria isolation method on medium, Gram staning, catalase test, spore test, motility test, TSIA, mannitol and glucose test. 25 samples form the cervical mucus of HF dairy cattle were collected in plastic sheath, post artificial insemination. Out of 25 isolate samples, 19 were Gram positive bacteria, 10 were cocobacil Gram negative bacteria, 7 samples of coccus Gram positive bacteria, while 3 samples did not show any growth. Non specific bacteria identified were Corynebacterium genus 17/25 (68%), Escherichia genus 10/25 (40%) and Staphylococcus genus 6/25 (24%).