Screening of Selected Stingless Bee Honey Varieties for ACE2-Spike Protein-Binding Inhibition Activity: A Potential Preventive Medicine Against SARS-Cov-2 Infection

Enos Tangke Arung; Rico Ramadhan; Liidza Diana Mandzilkh; Prasetia Aktavinaldy Santoso; Masako Matsumoto; Maki Nagata; Irawan Wijaya Kusuma; Swandari Paramita; Sukemi; Yadi; Naomichi Takemoto; Yhiya Amen; Syafrizal; Kuniyoshi Shimizu; Nataniel Tandirogang

doi:10.21315/mjms2022.29.5.15

Screening of Selected Stingless Bee Honey Varieties for ACE2-Spike Protein-Binding Inhibition Activity: A Potential Preventive Medicine Against SARS-Cov-2 Infection

Enos Tangke Arung, Rico Ramadhan, Liidza Diana Mandzilkh, Prasetia Aktavinaldy Santoso, Masako Matsumoto, Maki Nagata, Irawan Wijaya Kusuma, Swandari Paramita, Sukemi, Yadi, Naomichi Takemoto, Yhiya Amen, Syafrizal, Kuniyoshi Shimizu, Nataniel Tandirogang

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

Abstract

The broader objective of this study is to identify natural materials that might inhibit the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection. We have focused on stingless bee honey, which has a unique taste that is both sweet and sour and sometimes bitter. We screened 12 samples of honey from 11 species of stingless bees using an angiotensin-converting enzyme 2 (ACE2)-spike protein-binding assay and phytochemical analysis. Ten of the samples showed inhibition above 50% in this assay system. Most of the honey contained tannins, alkaloids, flavonoids, triterpenoids, carotenoids and carbohydrates. Our findings in this in vitro study showed that honey from stingless bees may have a potent effect against SARS-CoV-2 infection by inhibiting the ACE2-spike protein-binding.

Original language	English
Pages (from-to)	146-153
Number of pages	8
Journal	Malaysian Journal of Medical Sciences
Volume	29
Issue number	5
DOIs	https://doi.org/10.21315/mjms2022.29.5.15
Publication status	Published - 2022

Keywords

ACE2-spike protein-binding
SARS-CoV-2
honey
phytochemical
stingless bee

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10.21315/mjms2022.29.5.15

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Arung, E. T., Ramadhan, R., Mandzilkh, L. D., Santoso, P. A., Matsumoto, M., Nagata, M., Kusuma, I. W., Paramita, S., Sukemi, Yadi, Takemoto, N., Amen, Y., Syafrizal, Shimizu, K., & Tandirogang, N. (2022). Screening of Selected Stingless Bee Honey Varieties for ACE2-Spike Protein-Binding Inhibition Activity: A Potential Preventive Medicine Against SARS-Cov-2 Infection. Malaysian Journal of Medical Sciences, 29(5), 146-153. https://doi.org/10.21315/mjms2022.29.5.15

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title = "Screening of Selected Stingless Bee Honey Varieties for ACE2-Spike Protein-Binding Inhibition Activity: A Potential Preventive Medicine Against SARS-Cov-2 Infection",

abstract = "The broader objective of this study is to identify natural materials that might inhibit the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection. We have focused on stingless bee honey, which has a unique taste that is both sweet and sour and sometimes bitter. We screened 12 samples of honey from 11 species of stingless bees using an angiotensin-converting enzyme 2 (ACE2)-spike protein-binding assay and phytochemical analysis. Ten of the samples showed inhibition above 50% in this assay system. Most of the honey contained tannins, alkaloids, flavonoids, triterpenoids, carotenoids and carbohydrates. Our findings in this in vitro study showed that honey from stingless bees may have a potent effect against SARS-CoV-2 infection by inhibiting the ACE2-spike protein-binding.",

keywords = "ACE2-spike protein-binding, SARS-CoV-2, honey, phytochemical, stingless bee",

author = "Arung, {Enos Tangke} and Rico Ramadhan and Mandzilkh, {Liidza Diana} and Santoso, {Prasetia Aktavinaldy} and Masako Matsumoto and Maki Nagata and Kusuma, {Irawan Wijaya} and Swandari Paramita and Sukemi and Yadi and Naomichi Takemoto and Yhiya Amen and Syafrizal and Kuniyoshi Shimizu and Nataniel Tandirogang",

year = "2022",

doi = "10.21315/mjms2022.29.5.15",

language = "English",

volume = "29",

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Arung, ET, Ramadhan, R, Mandzilkh, LD, Santoso, PA, Matsumoto, M, Nagata, M, Kusuma, IW, Paramita, S, Sukemi, Yadi, Takemoto, N, Amen, Y, Syafrizal, Shimizu, K & Tandirogang, N 2022, 'Screening of Selected Stingless Bee Honey Varieties for ACE2-Spike Protein-Binding Inhibition Activity: A Potential Preventive Medicine Against SARS-Cov-2 Infection', Malaysian Journal of Medical Sciences, vol. 29, no. 5, pp. 146-153. https://doi.org/10.21315/mjms2022.29.5.15

Screening of Selected Stingless Bee Honey Varieties for ACE2-Spike Protein-Binding Inhibition Activity: A Potential Preventive Medicine Against SARS-Cov-2 Infection. / Arung, Enos Tangke; Ramadhan, Rico; Mandzilkh, Liidza Diana et al.
In: Malaysian Journal of Medical Sciences, Vol. 29, No. 5, 2022, p. 146-153.

Research output: Contribution to journal › Article › peer-review

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T1 - Screening of Selected Stingless Bee Honey Varieties for ACE2-Spike Protein-Binding Inhibition Activity

T2 - A Potential Preventive Medicine Against SARS-Cov-2 Infection

AU - Arung, Enos Tangke

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AU - Mandzilkh, Liidza Diana

AU - Santoso, Prasetia Aktavinaldy

AU - Matsumoto, Masako

AU - Nagata, Maki

AU - Kusuma, Irawan Wijaya

AU - Paramita, Swandari

AU - Sukemi,

AU - Yadi,

AU - Takemoto, Naomichi

AU - Amen, Yhiya

AU - Syafrizal,

AU - Shimizu, Kuniyoshi

AU - Tandirogang, Nataniel

PY - 2022

Y1 - 2022

N2 - The broader objective of this study is to identify natural materials that might inhibit the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection. We have focused on stingless bee honey, which has a unique taste that is both sweet and sour and sometimes bitter. We screened 12 samples of honey from 11 species of stingless bees using an angiotensin-converting enzyme 2 (ACE2)-spike protein-binding assay and phytochemical analysis. Ten of the samples showed inhibition above 50% in this assay system. Most of the honey contained tannins, alkaloids, flavonoids, triterpenoids, carotenoids and carbohydrates. Our findings in this in vitro study showed that honey from stingless bees may have a potent effect against SARS-CoV-2 infection by inhibiting the ACE2-spike protein-binding.

AB - The broader objective of this study is to identify natural materials that might inhibit the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection. We have focused on stingless bee honey, which has a unique taste that is both sweet and sour and sometimes bitter. We screened 12 samples of honey from 11 species of stingless bees using an angiotensin-converting enzyme 2 (ACE2)-spike protein-binding assay and phytochemical analysis. Ten of the samples showed inhibition above 50% in this assay system. Most of the honey contained tannins, alkaloids, flavonoids, triterpenoids, carotenoids and carbohydrates. Our findings in this in vitro study showed that honey from stingless bees may have a potent effect against SARS-CoV-2 infection by inhibiting the ACE2-spike protein-binding.

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SN - 1394-195X

VL - 29

SP - 146

EP - 153

JO - Malaysian Journal of Medical Sciences

JF - Malaysian Journal of Medical Sciences

IS - 5

ER -

Screening of Selected Stingless Bee Honey Varieties for ACE2-Spike Protein-Binding Inhibition Activity: A Potential Preventive Medicine Against SARS-Cov-2 Infection

Abstract

Keywords

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