Screening and identifying of cellulolytic bacteria from Alas Purwo National Park

Syahriar Nur Maulana Malik Ibrahim; Sibyandhita Erhaa Amalia; Bunga Agatha; Mustikayanti Yuni Lestari; Antonius Dwi Saputra; Nur Laily Fachira Ikmala; Fazareyna Hyuwan Argi Ryanto; Puspita Ayu Dewi; Mochamad Khoirul Anam; Farah Aisyah Nafidiastri; Nastiti Trikurniadewi; Ditta Putri Kumalasari; Erta Tri; Gendut Hariyanto; Suwarni; Agus Supriyanto

doi:10.1063/1.5050160

Screening and identifying of cellulolytic bacteria from Alas Purwo National Park

Syahriar Nur Maulana Malik Ibrahim, Sibyandhita Erhaa Amalia, Bunga Agatha, Mustikayanti Yuni Lestari, Antonius Dwi Saputra, Nur Laily Fachira Ikmala, Fazareyna Hyuwan Argi Ryanto, Puspita Ayu Dewi, Mochamad Khoirul Anam, Farah Aisyah Nafidiastri, Nastiti Trikurniadewi, Ditta Putri Kumalasari, Erta Tri, Gendut Hariyanto, Suwarni, Agus Supriyanto, Nimatuzahroh

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

3 Citations (Scopus)

Abstract

This research aimed to screen and identifies cellulolytic bacteria from Alas Purwo National Park. Soil samples were taken from five points in each location by indirect sampling method. Bacteria were isolated with CMC (Carboxymethylcellulose) agar. The result had successfully obtained 16 isolates which produced cellulase. The cellulase activity index assay of bacteria used CMC agar modified with adds 1 % YME (Yeast Malt Extract) and then was incubated during one day at room temperature. Bacterial colonies were soaked with Congo red 1 % and rinsed with NaOH 10 % to visualize the halo zone formed. Three isolates with highest cellulase activity index were identified by observing the colony morphology, cell morphology, and the biochemical characters. The results of this research showed that the three highest potential isolates producing cellulase were BPA-B, BSA-D, and BPA-A with cellulase activity indexes respectively 10.67, 9.86, and 8.16. BPA-B, BSA-D, and BPA-A each have the similarity with genera Pimelobacter, Micrococcus, and Cellulomonas.

Original language	English
Title of host publication	Inventing Prosperous Future through Biological Research and Tropical Biodiversity Management
Subtitle of host publication	Proceedings of the 5th International Conference on Biological Science
Editors	Zuliyati Rohmah, Lisna Hidayati, Tuty Arisuryanti, Maryani, Ganies Riza Aristya
Publisher	American Institute of Physics Inc.
ISBN (Print)	9780735417182
DOIs	https://doi.org/10.1063/1.5050160
Publication status	Published - 15 Aug 2018
Event	5th International Conference on Biological Science: Inventing Prosperous Future through Biological Research and Tropical Biodiversity Management, ICBS 2017 - Yogyakarta, Indonesia Duration: 15 Sept 2017 → 16 Sept 2017

Publication series

Name	AIP Conference Proceedings
Volume	2002
ISSN (Print)	0094-243X
ISSN (Electronic)	1551-7616

Conference

Conference	5th International Conference on Biological Science: Inventing Prosperous Future through Biological Research and Tropical Biodiversity Management, ICBS 2017
Country/Territory	Indonesia
City	Yogyakarta
Period	15/09/17 → 16/09/17

Keywords

Alas Purwo National Park
cellulase activity index
cellulolytic bacteria

Access to Document

10.1063/1.5050160

Cite this

Ibrahim, S. N. M. M., Amalia, S. E., Agatha, B., Lestari, M. Y., Saputra, A. D., Ikmala, N. L. F., Ryanto, F. H. A., Dewi, P. A., Anam, M. K., Nafidiastri, F. A., Trikurniadewi, N., Kumalasari, D. P., Tri, E., Hariyanto, G., Suwarni, Supriyanto, A., & Nimatuzahroh (2018). Screening and identifying of cellulolytic bacteria from Alas Purwo National Park. In Z. Rohmah, L. Hidayati, T. Arisuryanti, Maryani, & G. R. Aristya (Eds.), Inventing Prosperous Future through Biological Research and Tropical Biodiversity Management: Proceedings of the 5th International Conference on Biological Science Article 020064 (AIP Conference Proceedings; Vol. 2002). American Institute of Physics Inc.. https://doi.org/10.1063/1.5050160

Ibrahim, Syahriar Nur Maulana Malik ; Amalia, Sibyandhita Erhaa ; Agatha, Bunga et al. / Screening and identifying of cellulolytic bacteria from Alas Purwo National Park. Inventing Prosperous Future through Biological Research and Tropical Biodiversity Management: Proceedings of the 5th International Conference on Biological Science. editor / Zuliyati Rohmah ; Lisna Hidayati ; Tuty Arisuryanti ; Maryani ; Ganies Riza Aristya. American Institute of Physics Inc., 2018. (AIP Conference Proceedings).

@inproceedings{f0b3b6805f5e4700880d2b875e7f8143,

title = "Screening and identifying of cellulolytic bacteria from Alas Purwo National Park",

abstract = "This research aimed to screen and identifies cellulolytic bacteria from Alas Purwo National Park. Soil samples were taken from five points in each location by indirect sampling method. Bacteria were isolated with CMC (Carboxymethylcellulose) agar. The result had successfully obtained 16 isolates which produced cellulase. The cellulase activity index assay of bacteria used CMC agar modified with adds 1 % YME (Yeast Malt Extract) and then was incubated during one day at room temperature. Bacterial colonies were soaked with Congo red 1 % and rinsed with NaOH 10 % to visualize the halo zone formed. Three isolates with highest cellulase activity index were identified by observing the colony morphology, cell morphology, and the biochemical characters. The results of this research showed that the three highest potential isolates producing cellulase were BPA-B, BSA-D, and BPA-A with cellulase activity indexes respectively 10.67, 9.86, and 8.16. BPA-B, BSA-D, and BPA-A each have the similarity with genera Pimelobacter, Micrococcus, and Cellulomonas.",

keywords = "Alas Purwo National Park, cellulase activity index, cellulolytic bacteria",

author = "Ibrahim, {Syahriar Nur Maulana Malik} and Amalia, {Sibyandhita Erhaa} and Bunga Agatha and Lestari, {Mustikayanti Yuni} and Saputra, {Antonius Dwi} and Ikmala, {Nur Laily Fachira} and Ryanto, {Fazareyna Hyuwan Argi} and Dewi, {Puspita Ayu} and Anam, {Mochamad Khoirul} and Nafidiastri, {Farah Aisyah} and Nastiti Trikurniadewi and Kumalasari, {Ditta Putri} and Erta Tri and Gendut Hariyanto and Suwarni and Agus Supriyanto and Nimatuzahroh",

note = "Publisher Copyright: {\textcopyright} 2018 Author(s).; 5th International Conference on Biological Science: Inventing Prosperous Future through Biological Research and Tropical Biodiversity Management, ICBS 2017 ; Conference date: 15-09-2017 Through 16-09-2017",

year = "2018",

month = aug,

day = "15",

doi = "10.1063/1.5050160",

language = "English",

isbn = "9780735417182",

series = "AIP Conference Proceedings",

publisher = "American Institute of Physics Inc.",

editor = "Zuliyati Rohmah and Lisna Hidayati and Tuty Arisuryanti and Maryani and Aristya, {Ganies Riza}",

booktitle = "Inventing Prosperous Future through Biological Research and Tropical Biodiversity Management",

}

Ibrahim, SNMM, Amalia, SE, Agatha, B, Lestari, MY, Saputra, AD, Ikmala, NLF, Ryanto, FHA, Dewi, PA, Anam, MK, Nafidiastri, FA, Trikurniadewi, N, Kumalasari, DP, Tri, E, Hariyanto, G, Suwarni, , Supriyanto, A & Nimatuzahroh 2018, Screening and identifying of cellulolytic bacteria from Alas Purwo National Park. in Z Rohmah, L Hidayati, T Arisuryanti, Maryani & GR Aristya (eds), Inventing Prosperous Future through Biological Research and Tropical Biodiversity Management: Proceedings of the 5th International Conference on Biological Science., 020064, AIP Conference Proceedings, vol. 2002, American Institute of Physics Inc., 5th International Conference on Biological Science: Inventing Prosperous Future through Biological Research and Tropical Biodiversity Management, ICBS 2017, Yogyakarta, Indonesia, 15/09/17. https://doi.org/10.1063/1.5050160

Screening and identifying of cellulolytic bacteria from Alas Purwo National Park. / Ibrahim, Syahriar Nur Maulana Malik; Amalia, Sibyandhita Erhaa; Agatha, Bunga et al.
Inventing Prosperous Future through Biological Research and Tropical Biodiversity Management: Proceedings of the 5th International Conference on Biological Science. ed. / Zuliyati Rohmah; Lisna Hidayati; Tuty Arisuryanti; Maryani; Ganies Riza Aristya. American Institute of Physics Inc., 2018. 020064 (AIP Conference Proceedings; Vol. 2002).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

TY - GEN

T1 - Screening and identifying of cellulolytic bacteria from Alas Purwo National Park

AU - Ibrahim, Syahriar Nur Maulana Malik

AU - Amalia, Sibyandhita Erhaa

AU - Agatha, Bunga

AU - Lestari, Mustikayanti Yuni

AU - Saputra, Antonius Dwi

AU - Ikmala, Nur Laily Fachira

AU - Ryanto, Fazareyna Hyuwan Argi

AU - Dewi, Puspita Ayu

AU - Anam, Mochamad Khoirul

AU - Nafidiastri, Farah Aisyah

AU - Trikurniadewi, Nastiti

AU - Kumalasari, Ditta Putri

AU - Tri, Erta

AU - Hariyanto, Gendut

AU - Suwarni,

AU - Supriyanto, Agus

AU - Nimatuzahroh,

PY - 2018/8/15

Y1 - 2018/8/15

N2 - This research aimed to screen and identifies cellulolytic bacteria from Alas Purwo National Park. Soil samples were taken from five points in each location by indirect sampling method. Bacteria were isolated with CMC (Carboxymethylcellulose) agar. The result had successfully obtained 16 isolates which produced cellulase. The cellulase activity index assay of bacteria used CMC agar modified with adds 1 % YME (Yeast Malt Extract) and then was incubated during one day at room temperature. Bacterial colonies were soaked with Congo red 1 % and rinsed with NaOH 10 % to visualize the halo zone formed. Three isolates with highest cellulase activity index were identified by observing the colony morphology, cell morphology, and the biochemical characters. The results of this research showed that the three highest potential isolates producing cellulase were BPA-B, BSA-D, and BPA-A with cellulase activity indexes respectively 10.67, 9.86, and 8.16. BPA-B, BSA-D, and BPA-A each have the similarity with genera Pimelobacter, Micrococcus, and Cellulomonas.

AB - This research aimed to screen and identifies cellulolytic bacteria from Alas Purwo National Park. Soil samples were taken from five points in each location by indirect sampling method. Bacteria were isolated with CMC (Carboxymethylcellulose) agar. The result had successfully obtained 16 isolates which produced cellulase. The cellulase activity index assay of bacteria used CMC agar modified with adds 1 % YME (Yeast Malt Extract) and then was incubated during one day at room temperature. Bacterial colonies were soaked with Congo red 1 % and rinsed with NaOH 10 % to visualize the halo zone formed. Three isolates with highest cellulase activity index were identified by observing the colony morphology, cell morphology, and the biochemical characters. The results of this research showed that the three highest potential isolates producing cellulase were BPA-B, BSA-D, and BPA-A with cellulase activity indexes respectively 10.67, 9.86, and 8.16. BPA-B, BSA-D, and BPA-A each have the similarity with genera Pimelobacter, Micrococcus, and Cellulomonas.

KW - Alas Purwo National Park

KW - cellulase activity index

KW - cellulolytic bacteria

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U2 - 10.1063/1.5050160

DO - 10.1063/1.5050160

M3 - Conference contribution

AN - SCOPUS:85052383791

SN - 9780735417182

T3 - AIP Conference Proceedings

BT - Inventing Prosperous Future through Biological Research and Tropical Biodiversity Management

A2 - Rohmah, Zuliyati

A2 - Hidayati, Lisna

A2 - Arisuryanti, Tuty

A2 - Maryani, null

A2 - Aristya, Ganies Riza

PB - American Institute of Physics Inc.

T2 - 5th International Conference on Biological Science: Inventing Prosperous Future through Biological Research and Tropical Biodiversity Management, ICBS 2017

Y2 - 15 September 2017 through 16 September 2017

ER -

Ibrahim SNMM, Amalia SE, Agatha B, Lestari MY, Saputra AD, Ikmala NLF et al. Screening and identifying of cellulolytic bacteria from Alas Purwo National Park. In Rohmah Z, Hidayati L, Arisuryanti T, Maryani, Aristya GR, editors, Inventing Prosperous Future through Biological Research and Tropical Biodiversity Management: Proceedings of the 5th International Conference on Biological Science. American Institute of Physics Inc. 2018. 020064. (AIP Conference Proceedings). doi: 10.1063/1.5050160

Screening and identifying of cellulolytic bacteria from Alas Purwo National Park

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