Screening and characterization of phosphate solubilizing bacteria from isolate of thermophilic bacteria

Evy Yulianti, Anna Rakhmawati

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

7 Citations (Scopus)

Abstract

The aims of this study were to select bacteria that has the ability to dissolve phosphate from thermophilic bacteria isolates after the Merapi eruption. Five isolates of selected bacteria was characterized and continued with identification. Selection was done by using a pikovskaya selective medium. Bacterial isolates were grown in selective medium and incubated for 48 hours at temperature of 55 ° C. Characterization was done by looking at the cell and colony morphology, physiological and biochemical properties. Identification was done with the Profile Matching method based on the reference genus Oscillospira traced through Bergey's Manual of Determinative Bacteriology. Dendogram was created based on similarity index SSM. The results showed there were 14 isolates of bacteria that were able to dissolve phosphate indicated by a clear zone surrounding the bacterial colony on selective media. Five isolates were selected with the largest clear zone. Isolates D79, D92, D110a, D135 and D75 have different characters. The result of phenotypic characters identification with Genus Oscillospira profile has a percentage of 100% similarity to isolate D92 and D110a; 92.31% for isolates D79, and 84.6% for isolates D75 and D135. Dendogram generated from average linkage algorithm / UPGMA using the Simple Matching Coefficient (SSM) algorithms showed, isolate thermophilic bacteria D75 and D135 are combined together to form cluster 1. D110a and D92 form a sub cluster A.

Original languageEnglish
Title of host publication4th International Conference on Research, Implementation, and Education of Mathematics and Science, 4TH ICRIEMS
Subtitle of host publicationResearch and Education for Developing Scientific Attitude in Sciences and Mathematics
EditorsSlamet Suyanto, Cahyorini Kusumawardani, Insih Wilujeng, Agus Maman Abadi
PublisherAmerican Institute of Physics Inc.
ISBN (Electronic)9780735415485
DOIs
Publication statusPublished - 4 Aug 2017
Externally publishedYes
Event4th International Conference on Research, Implementation, and Education of Mathematics and Sciences: Research and Education for Developing Scientific Attitude in Sciences and Mathematics, ICRIEMS 2017 - Yogyakarta, Indonesia
Duration: 15 May 201716 May 2017

Publication series

NameAIP Conference Proceedings
Volume1868
ISSN (Print)0094-243X
ISSN (Electronic)1551-7616

Conference

Conference4th International Conference on Research, Implementation, and Education of Mathematics and Sciences: Research and Education for Developing Scientific Attitude in Sciences and Mathematics, ICRIEMS 2017
Country/TerritoryIndonesia
CityYogyakarta
Period15/05/1716/05/17

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