Safflower (Carthamus tinctorius Linn.) Inhibits Cell Proliferation and Induces Apoptotic in Breast Cancer Cell Lines T47D

Adryan Fristiohady, Wirhamsah Al-Ramadan, La Ode Muhammad Fitrawan, Rini Hamsidi, La Ode Muhammad Julian Purnama, Muhammad Hajrul Malaka, Lidya Agriningsih Haruna, Sahidin

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

Background and Objective: Safflower (Carthamus tinctorius Linn.) is one of the medicinal plants that contain secondary metabolites that have the potential to as anti-cancer by inducing apoptosis. This study aims to determine the content of secondary metabolite compounds and the induction activity of apoptosis from ethanol extract of safflower in the T47D breast cancer cell line. Materials and Methods: Safflower was extracted using 96% ethanol and assayed for phytochemical screening, cytotoxic tests by cell counting kit-8 to determine inhibitory concentration and apoptosis induction activity by flow cytometry to determine the ability of samples induce the programmed cell cancer in death. The data collected was analyzed with the PRISM GraphPad version. Results: The ethanol extract of safflower contains flavonoid compounds, alkaloids, saponins, tannins and terpenoids. The results of the anticancer activity test showed an IC50 value of 479 µg mLG1 and the best percentage of apoptosis at a concentration of 200 µg mLG1 was 16.61% at the beginning of apoptosis and 10.52% at the end of apoptosis. Conclusion: The safflower can be developed as a breast anticancer agent that works through the induction of apoptosis to improve the effectiveness of breast cancer treatment.

Original languageEnglish
Pages (from-to)427-433
Number of pages7
JournalPakistan Journal of Biological Sciences
Volume26
Issue number8
DOIs
Publication statusPublished - 2023

Keywords

  • IC
  • Safflower
  • T47D cell line
  • apoptosis
  • breast cancer
  • flow cytometry

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