TY - JOUR
T1 - Safflower (Carthamus tinctorius Linn.) Inhibits Cell Proliferation and Induces Apoptotic in Breast Cancer Cell Lines T47D
AU - Fristiohady, Adryan
AU - Al-Ramadan, Wirhamsah
AU - Fitrawan, La Ode Muhammad
AU - Hamsidi, Rini
AU - Purnama, La Ode Muhammad Julian
AU - Malaka, Muhammad Hajrul
AU - Haruna, Lidya Agriningsih
AU - Sahidin,
N1 - Publisher Copyright:
© 2023 Adryan Fristiohady et al.
PY - 2023
Y1 - 2023
N2 - Background and Objective: Safflower (Carthamus tinctorius Linn.) is one of the medicinal plants that contain secondary metabolites that have the potential to as anti-cancer by inducing apoptosis. This study aims to determine the content of secondary metabolite compounds and the induction activity of apoptosis from ethanol extract of safflower in the T47D breast cancer cell line. Materials and Methods: Safflower was extracted using 96% ethanol and assayed for phytochemical screening, cytotoxic tests by cell counting kit-8 to determine inhibitory concentration and apoptosis induction activity by flow cytometry to determine the ability of samples induce the programmed cell cancer in death. The data collected was analyzed with the PRISM GraphPad version. Results: The ethanol extract of safflower contains flavonoid compounds, alkaloids, saponins, tannins and terpenoids. The results of the anticancer activity test showed an IC50 value of 479 µg mLG1 and the best percentage of apoptosis at a concentration of 200 µg mLG1 was 16.61% at the beginning of apoptosis and 10.52% at the end of apoptosis. Conclusion: The safflower can be developed as a breast anticancer agent that works through the induction of apoptosis to improve the effectiveness of breast cancer treatment.
AB - Background and Objective: Safflower (Carthamus tinctorius Linn.) is one of the medicinal plants that contain secondary metabolites that have the potential to as anti-cancer by inducing apoptosis. This study aims to determine the content of secondary metabolite compounds and the induction activity of apoptosis from ethanol extract of safflower in the T47D breast cancer cell line. Materials and Methods: Safflower was extracted using 96% ethanol and assayed for phytochemical screening, cytotoxic tests by cell counting kit-8 to determine inhibitory concentration and apoptosis induction activity by flow cytometry to determine the ability of samples induce the programmed cell cancer in death. The data collected was analyzed with the PRISM GraphPad version. Results: The ethanol extract of safflower contains flavonoid compounds, alkaloids, saponins, tannins and terpenoids. The results of the anticancer activity test showed an IC50 value of 479 µg mLG1 and the best percentage of apoptosis at a concentration of 200 µg mLG1 was 16.61% at the beginning of apoptosis and 10.52% at the end of apoptosis. Conclusion: The safflower can be developed as a breast anticancer agent that works through the induction of apoptosis to improve the effectiveness of breast cancer treatment.
KW - IC
KW - Safflower
KW - T47D cell line
KW - apoptosis
KW - breast cancer
KW - flow cytometry
UR - http://www.scopus.com/inward/record.url?scp=85176589487&partnerID=8YFLogxK
U2 - 10.3923/pjbs.2023.427.433
DO - 10.3923/pjbs.2023.427.433
M3 - Article
C2 - 37937336
AN - SCOPUS:85176589487
SN - 1028-8880
VL - 26
SP - 427
EP - 433
JO - Pakistan Journal of Biological Sciences
JF - Pakistan Journal of Biological Sciences
IS - 8
ER -