TY - JOUR
T1 - RUNX2 and ALP expression in osteoblast cells exposed by PMMA-HAp combination
T2 - An in vitro study
AU - Darjanki, Claudia Michelle
AU - Prahasanti, Chiquita
AU - Fitria A, Eka
AU - Kusumawardani, Banun
AU - Wijaksana, I. Komang Evan
AU - Aljunaid, Mohammed
N1 - Publisher Copyright:
© 2023
PY - 2023/3/1
Y1 - 2023/3/1
N2 - Objective: To observe the expression of Runt-Related Transcription Factors 2 (RUNX2) and Alkaline Phosphatase (ALP) markers in osteoblast cell cultures exposed to Polymethylmethacrylate (PMMA) combined with hydroxyapatite (HAp) material to improve osteointegration of bone implants. Methods: Sample of PMMA and HAp materials with a mixture of PMMA with HAp made from limestone as natural source which processed through Balai Besar Keramik (HApBBK) in the first group and a mixture of PMMA with HAp made from bovine bone which processed through Good Manufacturing Practice (HApGMP) in the second group. Twenty-four fetal rat calvarie osteoblast cell cultures were randomly divided into 6 groups: 7- and 14-day control group, 7 and 14 days PMMA-HApGMP group, 7 and 14 days PMMA-HApBBK group. The expression of RUNX2 and ALP was seen by immunocytochemical examination. Result: The one-way ANOVA with a significance value of 0.000 (p < 0.05). There was an increase in RUNX2 and ALP expressions on both PMMA-HApBBK and PMMA-HApGMP groups on days 7 and 14 in osteoblast cell cultures. Conclusion: The PMMA-HApBBK and PMMA-HApGMP showed an increase in the RUNX2 and ALP expression in osteoblast cell cultures which indicates a potential increase of osseointegration of bone implants.
AB - Objective: To observe the expression of Runt-Related Transcription Factors 2 (RUNX2) and Alkaline Phosphatase (ALP) markers in osteoblast cell cultures exposed to Polymethylmethacrylate (PMMA) combined with hydroxyapatite (HAp) material to improve osteointegration of bone implants. Methods: Sample of PMMA and HAp materials with a mixture of PMMA with HAp made from limestone as natural source which processed through Balai Besar Keramik (HApBBK) in the first group and a mixture of PMMA with HAp made from bovine bone which processed through Good Manufacturing Practice (HApGMP) in the second group. Twenty-four fetal rat calvarie osteoblast cell cultures were randomly divided into 6 groups: 7- and 14-day control group, 7 and 14 days PMMA-HApGMP group, 7 and 14 days PMMA-HApBBK group. The expression of RUNX2 and ALP was seen by immunocytochemical examination. Result: The one-way ANOVA with a significance value of 0.000 (p < 0.05). There was an increase in RUNX2 and ALP expressions on both PMMA-HApBBK and PMMA-HApGMP groups on days 7 and 14 in osteoblast cell cultures. Conclusion: The PMMA-HApBBK and PMMA-HApGMP showed an increase in the RUNX2 and ALP expression in osteoblast cell cultures which indicates a potential increase of osseointegration of bone implants.
KW - ALP
KW - Bone graft
KW - Dental implant
KW - Hydroxyapatite
KW - Polymethylmethacrylate
KW - RUNX2
UR - http://www.scopus.com/inward/record.url?scp=85148719768&partnerID=8YFLogxK
U2 - 10.1016/j.jobcr.2023.02.007
DO - 10.1016/j.jobcr.2023.02.007
M3 - Article
AN - SCOPUS:85148719768
SN - 2212-4268
VL - 13
SP - 277
EP - 282
JO - Journal of Oral Biology and Craniofacial Research
JF - Journal of Oral Biology and Craniofacial Research
IS - 2
ER -