Reversin increase the plasticity of bone marrow-derived mesenchymal stem cell for generation of cardiomyocyte in vitro.

Budi S. Pikir, Helen Susilowati, Eryk Hendrianto, Fedik Abdulrantam

Research output: Contribution to journalArticlepeer-review

13 Citations (Scopus)

Abstract

Aim: to speed up transdifferentiation of bone marrow-derived stem cells into cardiomyocyte in vitro by inducing dedifferentiation of bone marrow-derived mesenchymal stem cell, before induction by 5-aza-2'-deoxycytidine into cardiomyocyte. Methods: two-three months old 2.5 kg weight adult male New Zealanad Rabbits were anesthezied with ether, thigh bones were excised, and bone marrow cells were obtained by aspiration. Results: in our experiments after 1 week of mesenchymal stem cell cultures, 20 nM reversin was given to induce dediferentiation and after 24 hours exposure with 9μM 5-aza-2-deoxycytidine, early phase of cardiomyocyte differentiation appeared as cultured cell were strongly positive for GATA-4 and weakly positive for MLC-2ά, although beating cardiomyocyte has not yet appeared at the end of experiment. These experiments also showed a marked CD34+ and c-kit+ gene expression on RT-PCR examination. Conclusion: reversine increase plasticity of bone marrow-derived mesenchymal stem cell to generate cardiomyocyte after 5-aza-2'-deoxycytidine induction. CD34+ and c-kit+ may be a marker for cardiomyocyte progenitor cells.

Original languageEnglish
Pages (from-to)23-27
Number of pages5
JournalActa medica Indonesiana
Volume44
Issue number1
Publication statusPublished - Jan 2012

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