Restriction fragment length polymorphism on growth hormone gene of madrasin crossbreed cattle

Ghea Aquatica Puteri, Budi Utomo, Roesno Darsono

Research output: Contribution to journalArticlepeer-review

Abstract

This research aims to identify GH gene polymorphism in cattle as a result of cross-breeding between Madura and Limousine cattle (as Madrasin cattle) using Restriction Fragment Length Polymorphism (RFLP). Blood samples of 14Madrasin cattle were taken for DNA isolation, DNA amplification with GH primers by using PCR-RFLP method. Restriction enzyme was AluI. The results suggested L allele frequency reached 0.96 while V allele reached 0.075. Therefore, LL genotype was acquired, amounting to 0.928, with LV amounting to 0.017. It can be concluded that there is a polymorphism in GH genes of Madrasin cattle (L and V alleles).

Original languageEnglish
Pages (from-to)23-36
Number of pages14
JournalIndian Veterinary Journal
Volume97
Issue number3
Publication statusPublished - Mar 2020

Keywords

  • Growth hormone gene
  • Madrasin cattle
  • PCR-RFLP
  • Polymorphism

Fingerprint

Dive into the research topics of 'Restriction fragment length polymorphism on growth hormone gene of madrasin crossbreed cattle'. Together they form a unique fingerprint.

Cite this